Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Narayana R. Isola"'
Publikováno v:
Rapid Communications in Mass Spectrometry. 17:532-537
The 5'-3' exonuclease activity of DNA polymerase was utilized in the polymerase chain reaction system to generate a specific signal concomitant with amplification. These signals were detected by matrix-assisted laser desorption/ionization time-of-fli
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::487e94e8c5bec7951baa86b1f2602da2
https://doi.org/10.1002/rcm.939
https://doi.org/10.1002/rcm.939
Publikováno v:
Analytical Chemistry. 73:809-812
Oligonucleotide ions have been detected using matrix-assisted laser desorption/ionization (MALDI) under nonresonant laser irradiation of the sample. When mass resolution was not limited by adduct attachment to the analyte ions, the nonresonant MALDI
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ace47ce36f9bc1477376aadefffd8081
https://doi.org/10.1021/ac001006+
https://doi.org/10.1021/ac001006+
Publikováno v:
Analytical Letters. 32:2583-2594
In the present study, conventional and synchronous luminescence (SL) were utilized to investigate spectral differences in normal and neoplastic cells. The synchronous fluorescence (SF) method involves scanning simultaneously both emission and excitat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b955c19d21df8bbebf56957f7e0f004b
https://doi.org/10.1080/00032719908542989
https://doi.org/10.1080/00032719908542989
Publikováno v:
Instrumentation Science & Technology. 26:503-514
This work involves the development and evaluation of a multiarray biosensor for DNA diagnostics. The evaluation of various system components developed for the biosensor is discussed. The DNA probes labeled with visible and near infrared (NIR) dyes ar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8d503a004f7d43c60ce9949ae34d4c0b
https://doi.org/10.1080/10739149808000917
https://doi.org/10.1080/10739149808000917
Autor:
David L. Cooper, Horng-Jyh Harn, Narayana R. Isola, Anil Datta, George Phillips, Edward W. Baptist, Jeffrey D. Byers, Suzanne Jackson, Graeme J. Dougherty
Publikováno v:
Biochemical and Biophysical Research Communications. 182:569-578
We have utilized the polymerase chain reaction (PCR) to isolate a 3.5 kilobase pair (kb) genomic fragment that encodes the additional extracellular domain unique to the epithelial isoform of CD44 (CD44E). Nucleotide sequence was determined for this c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb30948567d8798ae422e2e959b11b18
https://doi.org/10.1016/0006-291x(92)91770-q
https://doi.org/10.1016/0006-291x(92)91770-q
Publikováno v:
Gene. 98:201-207
Amino acid (aa) sequence data from Staphylococcus aureus V8 protease-digested bovine corneal 54-kDa protein (BCP54) fragments were utilized to derive mixed oligodeoxyribonucleotide (oligo) primers complementary to the reverse translation products of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64e076c191d7b0bc52bcb93add1a0fba
https://doi.org/10.1016/0378-1119(91)90174-a
https://doi.org/10.1016/0378-1119(91)90174-a
Autor:
Narayana R. Isola, David L. Cooper, Edward W. Baptist, Gordon K. Klintworth, Howard J. Lee, Jan J. Enghild
Publikováno v:
Current Eye Research. 9:781-786
The most abundant soluble protein of bovine cornea, BCP 54 (Bovine Corneal Protein, molecular weight 54 kD) was isolated and digested under both limited and complete digestion conditions with Staphylococcus aureus V8 protease. The fragments resulting
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f71d8fd94ebd864d8f49e50a213466f
https://doi.org/10.3109/02713689008999573
https://doi.org/10.3109/02713689008999573
Publikováno v:
Faraday discussions. 132
A protocol for binding cresyl fast violet (CFV), a SERS-active dye (label) containing an aromatic amino group with a modified oligomer having a carboxy derivatized thymidine moiety using carbodiimide coupling has been achieved for the first time. Cov
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18e9c1e63f6b6fd815b809dc8584e91a
https://pubmed.ncbi.nlm.nih.gov/16833124
https://pubmed.ncbi.nlm.nih.gov/16833124
PCR-Based Full-Length cDNA Cloning Utilizing the Universal-Adaptor/Specific DOS Primer-Pair Strategy
Autor:
David L. Cooper, Narayana R. Isola
Biological systems are often influenced by molecules that are neither present in vast quantities or easily purified to homogeneity from other cellular constituents. The development of simple, efficient molecular cloning systems coupled with the relat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b86d77c88a3a80658c992c61870d9624
https://doi.org/10.1385/0-89603-244-2:305
https://doi.org/10.1385/0-89603-244-2:305
Publikováno v:
Advanced Biomedical and Clinical Diagnostic Systems.
We describe the development of a surface-enhanced Raman scattering gene (SERGen) probe technology for rapid screening for diseases and pathogens through DNA hybridization assays. The technology combines the use of gene probes labeled with SERS-active
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0a946b957be4e99b07a1f93f26c52b8c
https://doi.org/10.1117/12.488679
https://doi.org/10.1117/12.488679