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Construction of Long-Transcript Enriched cDNA Libraries from Submicrogram Amounts of Total RNAs by a Universal PCR Amplification Method

Autor: Meng K. Lim, Minoru S.H. Ko, Yulan Piao, Naomi T. Ko

Publikováno v: Genome Research. 11:1553-1558

Here we report a novel design of linker primer that allows one to differentially amplify long tracts (average 3.0 kb with size ranges of 1–7 kb) or short DNAs (average 1.5 kb with size ranges of 0.5–3 kb) from a complex mixture. The method allows

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74937b44b8cf26fa5582533ece2cf4a6
https://doi.org/10.1101/gr.185501

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In Situ-Synthesized Novel Microarray Optimized for Mouse Stem Cell and Early Developmental Expression Profiling

Autor: Mark G. Carter, Pius M. Brzoska, Naomi T. Ko, Condie E. Carmack, Dawood B. Dudekula, Alexei A. Sharov, Toshio Hamatani, Yong Qian, Minoru S.H. Ko, S. Stuart Hwang, Kazuhiro Aiba

Applications of microarray technologies to mouse embryology/genetics have been limited, due to the nonavailability of microarrays containing large numbers of embryonic genes and the gap between microgram quantities of RNA required by typical microarr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21be7ac3cd92c1c3f384cb1a9b70e535
https://europepmc.org/articles/PMC430900/

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