Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Naoki Harikai"'
Publikováno v:
BioTechniques, Vol 73, Iss 6, Pp 281-287 (2022)
This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase. The extended DNA was used for real-time P
Externí odkaz:
https://doaj.org/article/5d89c9131e0d4518a724bc4c0a12bf1d
Autor:
Naoki Harikai, Yoichiro Ito, Yukina Harada, Kazufusa Shinomiya, Koji Tokura, Miho Yasue, Kazumasa Zaima
Publikováno v:
Journal of Chromatography A. 1481:64-72
Coil satellite centrifuge (CSC) produces the complex satellite motion consisting of the triplicate rotation of the coiled column around three axes including the sun axis (the angular velocity, ω1), the planet axis (ω2) and the satellite axis (the c
Autor:
Naoki Harikai, Naoki Sugimoto, Yusai Ito, Kyoko Ishizuki, Hiroshi Akiyama, Kazufusa Shinomiya
Publikováno v:
CHEMICAL & PHARMACEUTICAL BULLETIN. 65:883-887
Cochineal extract prepared from the scale insect Dactylopus coccus (American cochineal) has been used as a natural red dye for food, cosmetics, and pharmaceuticals. The major pigment in cochineal extract is carminic acid (CA), an anthraquinone glucos
Autor:
Yoshiyuki Sakuma, Rika Honda, Kazumasa Zaima, Naoki Harikai, Miho Yasue, Yoichiro Ito, Kazufusa Shinomiya, Koji Tokura
Publikováno v:
J Chromatogr A
The partition efficiencies of three different coiled columns, conventional multilayer coiled column, eccentric coiled column and toroidal coiled column, were evaluated by the separation of 4-methylumbelliferyl sugar derivatives using the coil satelli
Autor:
Naoki Sugimoto, Hiroshi Akiyama, Yusai Ito, Naoki Harikai, Ryoko Yagi, Kazumasa Zaima, Azusa Fukamachi, Kazufusa Shinomiya
Publikováno v:
Chemicalpharmaceutical bulletin. 65(3)
Carminic acid (CA) is a major component of cochineal dye used in food additives, cosmetics, and pharmaceuticals. CA and its isomers, 2-C-α-glucofuranoside and 2-C-β-glucofuranoside of kermesic acid (DCIV and DCVII, respectively), were isolated from
Autor:
Kazufusa Shinomiya, Naoki Harikai
Publikováno v:
Food Analytical Methods. 8:1215-1224
To improve the efficiency of mitochondrial DNA (mtDNA) extraction from food, a simple method, PrepM, was developed by effectively combining alkaline sodium dodecyl sulfate lysis and DNA binding to a silica membrane. PrepM was evaluated using fresh, c
Autor:
Manami Seki, Kazufusa Shinomiya, Jun Nitta, Motoki Umezawa, Yoichiro Ito, Naoki Harikai, Kazumasa Zaima
Publikováno v:
Separations; Volume 3; Issue 4; Pages: 29
(1) Background: Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of peak resolution and shortening of separation time. (2) Methods: The lon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29796cc97f456a1448de9d6adfa20cfc
https://europepmc.org/articles/PMC5119458/
https://europepmc.org/articles/PMC5119458/
Autor:
Reiko Teshima, Naoki Harikai, Kosuke Nakamura, Hiroshi Akiyama, Kazumi Kitta, Osamu Nakajima, Kazuhiko Minematsu, Tayoshi Iizuka
Publikováno v:
Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi). 51:247-252
Rice powder containing Konjak flour made with tuberous roots of Amorphophallus konjac is imported as a rice-processed product from China to Japan. An improved DNA purification method for the polymerase chain reaction (PCR) analysis of rice in such pr
Publikováno v:
International Journal of Food Science & Technology. 44:1778-1785
Summary We developed a method using an internal standard to determine the amount of unprocessed genetically modified organisms (GMO) in foods as GMO weight per weight of total food (w/wtf) using real-time PCR. Food samples were spiked with an interna
Autor:
Hiroshi Akiyama, Kazumi Kitta, Midori Abe, Reiko Teshima, Naoki Harikai, Kazunari Kondo, Kenji Kinoshita, Shin Saito
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 73:1886-1889
A novel DNA microarray method to detect one line of genetically modified (GM) soybean and five lines of GM maize was developed using multiplex PCR coupled with primer extension on a plastic plate. Multiplex PCR products were applied on an extension p