Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Naohiro Wako"'
Autor:
Hiroshi Nagashima, Takashi Ochiai, Koichiro Kano, Naohiro Wako, Mayuko Kurome, Hitomi Matsunari, Ryo Tomii
Publikováno v:
Journal of Reproduction and Development. 55:121-127
Four methods of cell cycle synchronization of porcine preadipocytes for use as nuclear donors in somatic cell cloning were compared: serum starvation, differentiation induction, contact inhibition and roscovitine treatment. After three days of differ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b68618fb1bdadb8e172a0d84e69fdcc7
https://doi.org/10.1262/jrd.20126
https://doi.org/10.1262/jrd.20126
Autor:
Mayuko Kurome, Takashi Ochiai, Tatsuya Fujimura, Hiroshi Nagashima, Naohiro Wako, Koji Miyazaki, Yoichi Takahagi, Hiroshi Murakami
Publikováno v:
Cloning and Stem Cells. 5:367-378
This paper methodologically compares the electro-fusion (EF) and intracytoplasmic injection (ICI) methods, as well as simultaneous fusion/activation (SA) and delayed activation (DA), in somatic nuclear transfer in pigs using fetal fibroblast cells. C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad37344c454e7cb5dc3b75fb022151bd
https://doi.org/10.1089/153623003772032862
https://doi.org/10.1089/153623003772032862
Autor:
Mayuko Kurome, Tatsuya Fujimura, Takashi Ochiai, Yoichi Takahagi, Naohiro Wako, Masaru Okabe, Hiroshi Nagashima, S Saito, R Esaki, Koichiro Kano, Hiroshi Murakami
Publikováno v:
Theriogenology. 59:95-106
Although pronuclear DNA micro-injection has long been the most reliable method to produce transgenic pigs, the efficiency of production of transgenic offspring is generally plagued by 1% of the DNA-injected embryos. Therefore, a problem with this met
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c4f879d6bb0b88ce6334dc8835dd1cf
https://doi.org/10.1016/s0093-691x(02)01261-x
https://doi.org/10.1016/s0093-691x(02)01261-x
Autor:
Hiroshi Murakami, Ken-ichi Mizuno, Koichiro Kano, Hiroshi Nagashima, Tatsuya Fujimura, Shuji Miyagawa, Takashi Kurihara, Mayuko Kurome, Takashi Ochiai, Naohiro Wako, Ryota Shirakura, Yoichi Takahagi
Publikováno v:
Journal of Reproduction and Development. 48:271-279
Developmental competence of in vitro matured porcine oocytes obtained by TCM199- and NCSU 23-based IVM systems after electrical activation were compared. When the nuclear phase of granulosa-cumulus-oocyte complexes (GCOCs) used in the TCM199-based IV
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d285534afc94f1533091ddd016ca13ae
https://doi.org/10.1262/jrd.48.271
https://doi.org/10.1262/jrd.48.271
Autor:
Takashi Kurihara, Ken-ichi Mizuno, Takashi Ochiai, Tatsuya Fujimura, Hiroshi Nagashima, Hiroshi Murakami, Naohiro Wako, Yoichi Takahagi, Mayuko Kurome
Publikováno v:
Journal of Reproduction and Development. 48:121-129
Porcine nuclear transfer embryos were reconstructed by intracytoplasmic nuclear injection of cumulus cell nuclei into recipient oocytes matured in vitro by two types of method (NCSU23-based and TCM199-based methods). Remodelling of the transferred nu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7f20d2ab5270da1ceabf066fcbe80907
https://doi.org/10.1262/jrd.48.121
https://doi.org/10.1262/jrd.48.121
Autor:
Ryo Tomii, Naohiro Wako, Mayuko Kurome, Koichiro Kano, Takashi Ochiai, Hideto Ueda, Kazumasa Hirakawa, Hiroshi Nagashima
Publikováno v:
Cloning and stem cells. 7(4)
The aim of the present study was to determine whether porcine preadipocytes can be efficient donor cells for somatic cell nuclear transfer (SCNT) in pigs. Primary culture of porcine preadipocytes was established by de-differentiating mature fat cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d88bde6a97aea4890edd702fa64b084d
https://pubmed.ncbi.nlm.nih.gov/16390264
https://pubmed.ncbi.nlm.nih.gov/16390264
Autor:
Ryo Tomii, Mayuko Kurome, Takashi Ochiai, Naohiro Wako, Hideto Ueda, Kazumasa Hirakawa, Koichiro Kano, Hiroshi Nagashima
Publikováno v:
Cloning & Stem Cells; Winter2005, Vol. 7 Issue 4, p279-288, 10p