Emergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant Saccharomyces cerevisiae under Glucose-Limited Conditions

Autor: Naia Risager Wright, Mathew M. Jessop-Fabre, Benjamin J. Sánchez, Tune Wulff, Christopher T. Workman, Nanna Petersen Rønnest, Nikolaus Sonnenschein

Publikováno v: Wright, N R, Jessop-Fabre, M M, Sánchez, B J, Wulff, T, Workman, C T, Rønnest, N P & Sonnenschein, N 2022, ' Emergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant Saccharomyces cerevisiae under Glucose-Limited Conditions ', Applied and Environmental Microbiology, vol. 88, no. 7, e0230721 . https://doi.org/10.1128/aem.02307-21
Appl Environ Microbiol

Cells cultured in a nutrient-limited environment can undergo adaptation, which confers improved fitness under long-term energy limitation. We have shown previously how a recombinant Saccharomyces cerevisiae strain, producing a heterologous insulin pr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6764ae392bf14cc9cd618e3658a432ba
https://doi.org/10.1128/aem.02307-21

Emergence of phenotypically distinct subpopulations is a factor in adaptation of recombinant Saccharomyces cerevisiae under glucose-limited conditions

Autor: Nanna Petersen Rønnest, Nikolaus Sonnenschein, Christopher T. Workman, Tune Wulff, Naia Risager Wright

Cells cultured in a nutrient-limited environment can undergo adaptation, which confers improved fitness under long-term energy limitation. We have previously shown how a recombinant S. cerevisiae strain, producing a heterologous insulin product, unde

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::649a21b70124872f3ff4f8fbe05350d1
https://doi.org/10.1101/2021.01.18.427050

Fluctuations in glucose availability prevent global proteome changes and physiological transition during prolonged chemostat cultivations of S. cerevisiae

Autor: Nanna Petersen Rønnest, Thomas Rubæk Jørgensen, Markus J. Herrgård, Nikolaus Sonnenschein, Naia Risager Wright, Eva Palmqvist, Christopher T. Workman, Tune Wulff

Publikováno v: Wright, N R, Wulff, T, Palmqvist, E A, Jørgensen, T R, Workman, C T, Sonnenschein, N, Rønnest, N P & Herrgård, M J 2020, ' Fluctuations in glucose availability prevent global proteome changes and physiological transition during prolonged chemostat cultivations of S. cerevisiae ', Biotechnology and Bioengineering, vol. 117, no. 7, pp. 2074-2088 . https://doi.org/10.1002/bit.27353

Chemostat cultivation mode imposes selective pressure on cells, which may result in slow adaptation in the physiological state over time. We applied a two-compartment scale-down chemostat system imposing feast-famine conditions to characterize the lo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a3d5f8cce43f331147e0fe5ecdabad9
https://orbit.dtu.dk/en/publications/2265a4d0-22a9-4b34-8947-780c7afb9404

Scale-down of continuous protein producingSaccharomyces cerevisiaecultivations using a two-compartment system

Autor: Jette Thykaer, Nanna Petersen Rønnest, Naia Risager Wright

Publikováno v: Biotechnology Progress. 32:152-159

In the biotechnological industry, economic decisions in investment are typically based on laboratory-scale experiments. Scale-down as a tool is therefore of high industrial importance to transfer the processes into larger production scale without los

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::176ef385f8302ffa729d69788cee1339
https://doi.org/10.1002/btpr.2184

Comparison of laser diffraction and image analysis for measurement of Streptomyces coelicolor cell clumps and pellets

Autor: Stuart M. Stocks, Nanna Petersen Rønnest, Anna Eliasson Lantz, Krist V. Gernaey

Publikováno v: Biotechnology Letters. 34:1465-1473

Morphology is important in industrial processes involving filamentous organisms because it affects the mixing and mass transfer and can be linked to productivity. Image analysis provides detailed information about the morphology but, in practice, it

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbc732ed6a51b795cd61a8839a6881ca
https://doi.org/10.1007/s10529-012-0936-1