Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Nanlan, Xu"'
Autor:
Ellen Nordman, Kaiqin Lao, M. Azim Surani, Vladimir I. Bashkirov, Catalin Barbacioru, Fuchou Tang, Bin Li, Nanlan Xu
Publikováno v:
Nature Protocols. 5:516-535
We describe here a protocol for digital transcriptome analysis in a single mouse blastomere using a deep sequencing approach. An individual blastomere was first isolated and put into lysate buffer by mouth pipette. Reverse transcription was then perf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f1f47673f7970150accae2f301e6fbd
https://doi.org/10.1038/nprot.2009.236
https://doi.org/10.1038/nprot.2009.236
Autor:
Clarence Lee, Xiaohui Wang, Asim Siddiqui, Brian B. Tuch, John Bodeau, Fuchou Tang, Catalin Barbacioru, Yangzhou Wang, Nanlan Xu, M. Azim Surani, Ellen Nordman, Kaiqin Lao
Publikováno v:
Nature Methods. 6:377-382
Next-generation sequencing technology is a powerful tool for transcriptome analysis. However, under certain conditions, only a small amount of material is available, which requires more sensitive techniques that can preferably be used at the single-c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f69748f0be9904a222b2b457e343bb37
https://doi.org/10.1038/nmeth.1315
https://doi.org/10.1038/nmeth.1315
Autor:
Kai Q, Lao, Fuchou, Tang, Catalin, Barbacioru, Yangzhou, Wang, Ellen, Nordman, Clarence, Lee, Nanlan, Xu, Xiaohui, Wang, Brain, Tuch, John, Bodeau, Asim, Siddiqui, M Azim, Surani
Publikováno v:
Journal of biomolecular techniques : JBT. 20(5)
We have developed a sequencing-based gene expression profiling assay at single-cell resolution by combining a modified single-cell whole transcriptome amplification method with the next generation sequencing technique, the SOLiD™ system. Using this
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3a4d31303be80bf3a4ce1242145c1cd0
https://pubmed.ncbi.nlm.nih.gov/19949700
https://pubmed.ncbi.nlm.nih.gov/19949700
Autor:
Fuchou Tang, Barbacioru, Catalin, Yangzhou Wang, Nordman, Ellen, Clarence Lee, Nanlan Xu, Xiaohui Wang, Bodeau, John, Tuch, Brian B, Siddiqui, Asim, Lao, Kaiqin, Surani, M. Azim
Publikováno v:
Nature Methods; May2009, Vol. 6 Issue 5, p377-382, 6p, 1 Diagram, 1 Chart, 4 Graphs