Zobrazeno 1 - 10
of 54
pro vyhledávání: '"Nancy L. Greenbaum"'
Publikováno v:
ACS Omega, Vol 5, Iss 37, Pp 23549-23558 (2020)
Externí odkaz:
https://doaj.org/article/6d46c710e6524419b927fc0d4f6ce485
Publikováno v:
RNA
U2 and U6 small nuclear (sn)RNAs are the only snRNAs directly implicated in catalyzing the splicing of pre-mRNA, but assembly and rearrangement steps prior to catalysis require numerous proteins. Previous studies have shown that the protein-free U2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ffb39084aeefc69ebe3a97130eb20c2
https://europepmc.org/articles/PMC7297123/
https://europepmc.org/articles/PMC7297123/
Autor:
Huong Chu, Naser Dodic, N. V. S. Dinesh K. Bhupathiraju, Julia Rozenberg, Nancy L. Greenbaum, Veronica Bueno, Charles Michael Drain, William Perea, Naxhije Berisha, Junior Gonzales
Publikováno v:
Chemical Communications. 53:3773-3776
A multifunctional chlorin platform appended with four short polyethylene glycols and a carboxylate-linker allows rapid conjugation to biotargeting motifs such as proteins and oligonucleotides. The stability and photophysical properties of the chlorin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bc7d87bdad8ce20580d41e0dc4caece
https://doi.org/10.1039/c7cc01265a
https://doi.org/10.1039/c7cc01265a
Publikováno v:
ACS Omega, Vol 5, Iss 37, Pp 23549-23558 (2020)
ACS Omega
ACS Omega
Splicing of precursor messenger RNA is catalyzed by the spliceosome, a dynamic ribonucleoprotein assembly composed of five small nuclear (sn)RNAs and >100 proteins. RNA components catalyze the two transesterification reactions, but proteins perform c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56285d1d249216dfc6c28d248da1085b
Autor:
Huong Chu, Nancy L. Greenbaum
Intron removal during splicing of precursor pre-mRNA requires assembly of spliceosomal small nuclear (sn)RNAs into catalytically competent conformations to promote two transesterification reactions. U2 and U6 snRNA are the only snRNAs directly implic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8b9a7a2f3c156d8f23450587ed835c7
https://doi.org/10.1101/825547
https://doi.org/10.1101/825547
Autor:
Nancy L. Greenbaum, William Perea
We describe a method to analyze the affinity and specificity of interactions between proteins and RNA using horizontal PAGE under non-denaturing conditions. The method permits tracking of migration of anionic and cationic biomolecules and complexes t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27be594fca4d4ef2f00f98d7cc0b685e
https://doi.org/10.1101/825679
https://doi.org/10.1101/825679
Publikováno v:
ACS nano. 10(2)
Optical ruler methods employing multiple fluorescent labels offer great potential for correlating distances among several sites, but are generally limited to interlabel distances under 10 nm and suffer from complications due to spectral overlap. Here
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de7b8915ca829481030156f61b8eab23
https://pubmed.ncbi.nlm.nih.gov/26795549
https://pubmed.ncbi.nlm.nih.gov/26795549
Publikováno v:
Biochemistry. 55(4)
We have probed the molecular basis of recognition between human spliceosomal U2 snRNP protein p14 and RNA targets representing the intron branch site region. Interaction of an RNA duplex representing the branch site helix perturbed at least 10 nuclea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3a1ce0950dbefb70ae756ed555d47a3
https://pubmed.ncbi.nlm.nih.gov/26784522
https://pubmed.ncbi.nlm.nih.gov/26784522
Publikováno v:
Nucleic Acids Research. 35:3836-3847
G.U wobble base pairs are the most common and highly conserved non-Watson-Crick base pairs in RNA. Previous surface maps imply uniformly negative electrostatic potential at the major groove of G.U wobble base pairs embedded in RNA helices, suitable f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5e016b7762ceaed25a3ba23394f88625
https://doi.org/10.1093/nar/gkm274
https://doi.org/10.1093/nar/gkm274
Autor:
LauraJane Phelps, Nancy L. Greenbaum, Volker Buschmann, Kenneth D. Weston, Laura M. Griffin, Faqing Yuan
Publikováno v:
Nucleic Acids Research
U2 and U6 snRNAs pair to form a phylogenetically conserved complex at the catalytic core of the spliceosome. Interactions with divalent metal ions, particularly Mg(II), at specific sites are essential for its folding and catalytic activity. We used a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70a5c94cf88b9ca50a131f0930f9d2be
https://doi.org/10.1093/nar/gkm134
https://doi.org/10.1093/nar/gkm134