Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Nancy L, Cowger"'
Publikováno v:
Enzyme and Microbial Technology. 31:600-608
Viscometers have had a prominent role in the study of hydrodynamic damage to cell cultures. A Couette bioreactor overcomes stringent time limits of previous viscometric research. At low shear levels, the vessel supported robust growth of Spodoptera f
Autor:
Mary K. Cheles, Kim C. O'Connor, Amy C. Primavera, Nancy L. Cowger, Salima Haque, Sanda Clejan
Publikováno v:
Biotechnology and Bioengineering. 50:587-597
The high aspect rotating-wall vessel (HARV) was recently designed by NASA to cultivate animal cells in an environment that simulates microgravity. This work examines the effects of HARV cultivation on DU 145 human prostate carcinoma cells. In the HAR
Publikováno v:
Biotechnology and Bioengineering. 64:14-26
In previous publications, we reported the benefits of a high-aspect rotating-wall vessel (HARV) over conventional bioreactors for insect-cell cultivation in terms of reduced medium requirements and enhanced longevity. To more fully understand the eff
Publikováno v:
Enzyme and Microbial Technology. 20:326-332
Simulated microgravity within the NASA High Aspect Rotating-Wall Vessel (HARV) provides a quiescent environment to culture fragile insect cells. In this vessel, the duration of stationary and death phase for cultures of Spodoptera frugiperda cells wa
Autor:
Lanika A, Brown, Linda M, Arterburn, Ana P, Miller, Nancy L, Cowger, Sonya M, Hartley, Annette, Andrews, Paul M, Silber, Albert P, Li
Publikováno v:
In vitro cellulardevelopmental biology. Animal. 39(1-2)
Rat hepatocytes were cultured initially as spheroids on culture plates and then transferred into a rotating wall vessel (high-aspect ratio vessel [HARV]) for further culturing. Morphological evaluation based on electron microscopy showed that hepatoc
Autor:
Linda M. Arterburn, Ana P. Miller, Sonya M. Hartley, Lanika A. Brown, Annette Andrews, Albert P. Li, Nancy L. Cowger, Paul M. Silber
Publikováno v:
In Vitro Cellular and Developmental Biology-Animal.
Rat hepatocytes were cultured initially as spheroids on culture plates and then transferred into a rotating wall vessel (high-aspect ratio vessel [HARV]) for further culturing. Morphological evaluation based on electron microscopy showed that hepatoc
Publikováno v:
Journal of applied physiology (Bethesda, Md. : 1985). 92(2)
The rotating wall vessel is optimized for suspension culture, with laminar flow and adequate nutrient delivery, but minimal shear. However, higher shears may occur in vivo. During rotating wall vessel cultivation of human renal cells, size and densit
Autor:
Salima Haque, Kim C. O'Connor, Robert S. Dotson, Mary K. Cheles, Nancy L. Cowger, Sanda Clejan, Richard M. Enmon
Publikováno v:
37th Aerospace Sciences Meeting and Exhibit.
Autor:
Nancy L. Cowger, Kim C. O'Connor
Publikováno v:
36th AIAA Aerospace Sciences Meeting and Exhibit.
There is a shift from necrosis to apoptosis when batch cultures of Spodoptera frugiperda are grown in the extreme low-shear regime of NASA's High-Aspect Rotating-Wall Vessel (HARV) versus a shaker flask. The NASA vessel was developed for the cultivat
Autor:
Lanika A. Brown, Paul M. Silber, Sonya M. Hartley, Ana T. Miller, Nancy L. Cowger, Albert P. Li
Publikováno v:
ASAIO Journal. 48:196