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Publikováno v:
BioTechniques, Vol 28, Iss 5, Pp 934-937 (2000)
A dot immunoblotting technique has been developed to estimate the relative expression levels of tagged recombinant human proteins in mammalian cell culture media. Variations in sample denaturation, blocking agents and membrane composition and treatme
Externí odkaz:
https://doaj.org/article/e30ac7d18dd642979f5d7bdc25ca38d4
Publikováno v:
BioTechniques. 28:934-937
A dot immunoblotting technique has been developed to estimate the relative expression levels of tagged recombinant human proteins in mammalian cell culture media. Variations in sample denaturation, blocking agents and membrane composition and treatme