Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Nana Jacobsen"'
Autor:
Peter Mouritzen, Peter S. Nielsen, Nana Jacobsen, Mikkel Noerholm, Christian Lomholt, Henrik M. Pfundheller, Niels B. Ramsing, Sakari Kauppinen, Niels Tolstrup
Publikováno v:
BioTechniques, Vol 37, Iss 3, Pp 492-495 (2004)
Externí odkaz:
https://doaj.org/article/487fcafed9f54bb0948a45fea409245a
Autor:
Mikkel Noerholm, Peter Stein Nielsen, Niels Tolstrup, Henrik M. Pfundheller, Christian Lomholt, Nana Jacobsen, Peter Mouritzen
Publikováno v:
Nature Methods. 2:313-316
ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described. The ProbeLibrary concept is based on the fact that just 90 short probes provide transcriptome-wide coverage in most organisms. These short probes are hig
Autor:
Mikkel Noerholm, Niels B. Ramsing, Nana Jacobsen, Sakari Kauppinen, Niels Tolstrup, Christian Lomholt, Peter Stein Nielsen, Peter Mouritzen, Henrik M. Pfundheller
Publikováno v:
BioTechniques. 37:492-495
While quantitative real-time RT-PCR is in principle a simple technique, the assay design remains fairly complex and designed assays often perform inadequately with respect to specificity, sensitivity, and reproducibility (1,2). The time spent on assa
Autor:
Søren Lind Rasmussen, Nana Jacobsen, Jef Fenstholt, Joan Bentzen, Mogens Fenger, Jan Skouv, Mogens Jakobsen
Publikováno v:
Clinical Chemistry. 48:657-660
Hyperlipidemia and coronary heart disease (CHD) are associated with genetic variation in the apolipoprotein B (apoB) gene (1). Nonexchangeable apolipoprotein B-100 (ApoB-100) is an important determinant of LDL-cholesterol in plasma; it plays a centra
Publikováno v:
Christensen, U, Jacobsen, N, Rajwanshi, V K, Wengel, J & Koch, T 2001, ' Stopped-flow kinetics of locked nucleic acid (LNA)-oligonucleotide duplex formation: studies of LNA-DNA and DNA-DNA interactions ', Biochemical Journal, vol. 354, no. 3, pp. 481-484 . https://doi.org/10.1042/bj3540481
The locked nucleic acid (LNA) monomer is a conformationally restricted nucleotide analogue with an extra 2'-O,4'-C-methylene bridge added to the ribose ring. Oligonucleotides that contain LNA monomers have shown greatly enhanced thermal stability whe
Publikováno v:
Bioconjugate Chemistry. 11:474-483
Ligand immobilization on solid surfaces is an essential step in fields such as diagnostics, bio sensor manufacturing, and new material sciences in general. In this paper a photochemical approach based on anthraquinone as the chromophore is presented.
Autor:
Mirella Ezban, Ole Thastrup, Donald C. Foster, Lars Christian Petersen, Nana Jacobsen, Brit B. Sørensen, Nils C.H. Bergenhem, Lars Kongsbak Poulsen, James D. Kelly
Publikováno v:
Journal of Biological Chemistry. 273:6228-6232
The putative role of tissue factor (TF) as a receptor involved in signal transduction is indicated by its sequence homology to cytokine receptors (Bazan, J. F. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 6934-6938). Signal transduction induced by bind
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 732
A variety of physiological processes are associated with changes in microRNA (miRNA) expression. Analysis of miRNA has been applied to study normal physiology as well as diseased states including cancer. One major challenge in miRNA research is to ac
Publikováno v:
MicroRNAs in Development ISBN: 9781617790829
A variety of physiological processes are associated with changes in microRNA (miRNA) expression. Analysis of miRNA has been applied to study normal physiology as well as diseased states including cancer. One major challenge in miRNA research is to ac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f199a64e5e410c1a495146dc9de0b99e
https://doi.org/10.1007/978-1-61779-083-6_4
https://doi.org/10.1007/978-1-61779-083-6_4
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 703
This chapter describes a method for the isolation of intact polyadenylated mRNA using LNA oligo(T) capture. The method enables efficient isolation of poly(A)(+) RNA directly from guanidinium thiocyanate (GuSCN)-containing cell or tissue extract by co