Validation of SARS-CoV-2 detection across multiple specimen types

Autor: Perchetti, Garrett A., Nalla, Arun K., Huang, Meei-Li, Zhu, Haiying, Wei, Yulun, Stensland, Larry, Loprieno, Michelle A., Jerome, Keith R., Greninger, Alexander L. ^*

Publikováno v: In Journal of Clinical Virology July 2020 128

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DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP.

Autor: Bruce EA; Department of Medicine, Division of Immunobiology, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA., Huang ML; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA., Perchetti GA; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA., Tighe S; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA., Laaguiby P; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA., Hoffman JJ; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA., Gerrard DL; Department of Pathology and Laboratory Medicine, University of Vermont Medical Center, Burlington VT, 05401, USA., Nalla AK; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA., Wei Y; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA., Greninger AL; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA 98109, USA., Diehl SA; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405 USA., Shirley DJ; IXIS LLC, Data Science Division, Burlington, VT 05401, USA., Leonard DGB; Department of Pathology and Laboratory Medicine, Robert Larner, M.D. College of Medicine, University of Vermont and the University of Vermont Health Network, Burlington VT, 05405, USA., Huston CD; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Department of Medicine, Division of Infectious Disease, University of Vermont Medical Center, Burlington VT, 05401, USA., Kirkpatrick BD; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405 USA.; Department of Medicine, Division of Infectious Disease, University of Vermont Medical Center, Burlington VT, 05401, USA., Dragon JA; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA., Crothers JW; Department of Pathology and Laboratory Medicine, Robert Larner, M.D. College of Medicine, University of Vermont and the University of Vermont Health Network, Burlington VT, 05405, USA., Jerome KR; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA 98109, USA., Botten JW; Department of Medicine, Division of Immunobiology, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405 USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2020 Apr 06. Date of Electronic Publication: 2020 Apr 06.