Triphenylphosphonium-modified catiomers enhance in vivo mRNA delivery through stabilized polyion complexation.

Autor: Norimatsu J; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. horacio@g.ecc.u-tokyo.ac.jp., Mizuno HL; Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan. anraku.y.aa@m.titech.ac.jp., Watanabe T; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. horacio@g.ecc.u-tokyo.ac.jp., Obara T; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. horacio@g.ecc.u-tokyo.ac.jp., Nakakido M; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. horacio@g.ecc.u-tokyo.ac.jp.; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan., Tsumoto K; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. horacio@g.ecc.u-tokyo.ac.jp.; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.; The Institute of Medical Sciences, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan., Cabral H; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. horacio@g.ecc.u-tokyo.ac.jp.; Innovation Center of NanoMedicine, Kawasaki Institute of Industrial Promotion, 3-25-14 Tonomachi, Kawasaki-ku, Kanagawa 210-0821, Japan., Kuroda D; Research Center of Drug and Vaccine Development, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. dkuroda@niid.go.jp., Anraku Y; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. horacio@g.ecc.u-tokyo.ac.jp.; Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan. anraku.y.aa@m.titech.ac.jp.; Innovation Center of NanoMedicine, Kawasaki Institute of Industrial Promotion, 3-25-14 Tonomachi, Kawasaki-ku, Kanagawa 210-0821, Japan.

Publikováno v: Materials horizons [Mater Horiz] 2024 Sep 30; Vol. 11 (19), pp. 4711-4721. Date of Electronic Publication: 2024 Sep 30.

Crystal structures of human CD40 in complex with monoclonal antibodies dacetuzumab and bleselumab.

Autor: Asano R; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan., Nakakido M; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan. Electronic address: nakakido@g.ecc.u-tokyo.ac.jp., Pérez JF; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan., Ise T; Laboratory of Antibody Design, Center for Drug Design Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan., Caaveiro JMM; Department of Protein Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan., Nagata S; Laboratory of Antibody Design, Center for Drug Design Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan., Tsumoto K; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan; Medical Device Development and Regulation Research Center, School of Engineering, The University of Tokyo, Tokyo, Japan; The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. Electronic address: tsumoto@bioeng.t.u-tokyo.ac.jp.

Publikováno v: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Jun 25; Vol. 714, pp. 149969. Date of Electronic Publication: 2024 Apr 18.

Characterization of a novel format scFv×VHH single-chain biparatopic antibody against metal binding protein MtsA.

Autor: Asano R; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan., Takeuchi M; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan., Nakakido M; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan.; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan., Ito S; Rigaku Corporation ROD Single Crystal Analysis Group Application Laboratories, Tokyo, Japan., Aikawa C; Section of Applied Veterinary Sciences, Division of Veterinary Sciences, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan., Yokoyama T; Graduate School of Life Sciences, Tohoku University, Miyagi, Japan.; The advanced center for innovations in next-generation medicine (INGEM), Tohoku University, Miyagi, Japan., Senoo A; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan., Ueno G; RIKEN SPring-8 Center, Hyogo, Japan., Nagatoishi S; Medical Device Development and Regulation Research Center, School of Engineering, The University of Tokyo, Tokyo, Japan., Tanaka Y; Graduate School of Life Sciences, Tohoku University, Miyagi, Japan.; The advanced center for innovations in next-generation medicine (INGEM), Tohoku University, Miyagi, Japan., Nakagawa I; Department of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan., Tsumoto K; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan.; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan.; Medical Device Development and Regulation Research Center, School of Engineering, The University of Tokyo, Tokyo, Japan.; The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

Publikováno v: Protein science : a publication of the Protein Society [Protein Sci] 2024 Jun; Vol. 33 (6), pp. e5017.

Functional insights of Tyr37 in framework region 2 directly contributing to the binding affinities and dissociation kinetics in single-domain V H H antibodies.

Autor: Yamamoto K; Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan., Nagatoishi S; The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan; Medical Device Development and Regulation Research Center, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. Electronic address: s-nagatoishi@g.ecc.u-tokyo.ac.jp., Nakakido M; Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan., Kuroda D; Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan., Tsumoto K; Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan; Medical Device Development and Regulation Research Center, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. Electronic address: tsumoto@bioeng.t.u-tokyo.ac.jp.

Publikováno v: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 May 21; Vol. 709, pp. 149839. Date of Electronic Publication: 2024 Mar 27.

Generation of antibodies to an extracellular region of the transporters Glut1/Glut4 by immunization with a designed antigen.

Autor: Sumikawa T; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan., Nakakido M; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan. Electronic address: nakakido@g.ecc.u-tokyo.ac.jp., Matsunaga R; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan., Kuroda D; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Tokyo, Japan., Nagatoishi S; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan; Laboratory of Medical Proteomics, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan., Tsumoto K; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan; Laboratory of Medical Proteomics, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. Electronic address: tsumoto@bioeng.t.u-tokyo.ac.jp.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2024 Feb; Vol. 300 (2), pp. 105640. Date of Electronic Publication: 2024 Jan 09.