Multiplexed targeted analysis of polyunsaturated fatty acids and oxylipins using liquid chromatography-tandem mass spectrometry

Autor: Evgenia Turtoi, Jeremy Jeudy, Gilles Valette, Christine Enjalbal, Isabelle K. Vila, Nadine Laguette, Andrei Turtoi

Publikováno v: STAR Protocols, Vol 4, Iss 3, Pp 102226- (2023)

Summary: Polyunsaturated fatty acids (PUFAs) and their oxidized products (oxylipins) are important mediators in intra- and extra-cellular signaling. We describe here the simultaneous quantification of 163 PUFAs and oxylipins using liquid chromatograp

Externí odkaz: https://doaj.org/article/57b01e3994af4369bd98639c345eec1f

Protocol to induce and assess cGAS-STING pathway activation in vitro

Autor: Hanane Chamma, Soumyabrata Guha, Nadine Laguette, Isabelle K. Vila

Publikováno v: STAR Protocols, Vol 3, Iss 2, Pp 101384- (2022)

Summary: The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway plays a pivotal role in several cellular processes including pathogen recognition and inflammatory responses. We describe a protocol to activate the cGAS-STING

Externí odkaz: https://doaj.org/article/ef12246d369b4bd4b372fcb30ebafb28

Nucleic Acid Immunity and DNA Damage Response: New Friends and Old Foes

Autor: Clara Taffoni, Alizée Steer, Johanna Marines, Hanane Chamma, Isabelle K. Vila, Nadine Laguette

Publikováno v: Frontiers in Immunology, Vol 12 (2021)

The maintenance of genomic stability in multicellular organisms relies on the DNA damage response (DDR). The DDR encompasses several interconnected pathways that cooperate to ensure the repair of genomic lesions. Besides their repair functions, sever

Externí odkaz: https://doaj.org/article/de91e91385b548d39ffaf0da95fd7a93

Phosphorylation of SAMHD1 by Cyclin A2/CDK1 Regulates Its Restriction Activity toward HIV-1

Autor: Alexandra Cribier, Benjamin Descours, Ana Luiza Chaves Valadão, Nadine Laguette, Monsef Benkirane

Publikováno v: Cell Reports, Vol 3, Iss 4, Pp 1036-1043 (2013)

SAMHD1 restricts HIV-1 replication in myeloid and quiescent CD4+ T cells. Here, we show that SAMHD1 restriction activity is regulated by phosphorylation. SAMHD1 interacts with cyclin A2/cdk1 only in cycling cells. Cyclin A2/CDK1 phosphorylates SAMHD1

Externí odkaz: https://doaj.org/article/2a73f623ac5248129fb0fa679570091d

Alternative pathways driven by STING: From innate immunity to lipid metabolism

Autor: Isabelle K. Vila, Soumyabrata Guha, Joanna Kalucka, David Olagnier, Nadine Laguette

Publikováno v: Vila, I K, Guha, S, Kalucka, J, Olagnier, D & Laguette, N 2022, ' Alternative pathways driven by STING : From innate immunity to lipid metabolism ', Cytokine and Growth Factor Reviews, vol. 68, pp. 54-68 . https://doi.org/10.1016/j.cytogfr.2022.08.006

The Stimulator of Interferon Genes (STING) is a major adaptor protein that is central to the initiation of type I interferon responses and proinflammatory signalling. STING-dependent signalling is triggered by the presence of cytosolic nucleic acids

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ceb0a4fce8fcabcd0c96dce01516e5a
https://doi.org/10.1016/j.cytogfr.2022.08.006