The P323L substitution in the SARS-CoV-2 polymerase (NSP12) confers a selective advantage during infection

Autor: Hannah Goldswain, Xiaofeng Dong, Rebekah Penrice-Randal, Muhannad Alruwaili, Ghada T. Shawli, Tessa Prince, Maia Kavanagh Williamson, Jayna Raghwani, Nadine Randle, Benjamin Jones, I’ah Donovan-Banfield, Francisco J. Salguero, Julia A. Tree, Yper Hall, Catherine Hartley, Maximilian Erdmann, James Bazire, Tuksin Jearanaiwitayakul, Malcolm G. Semple, Peter J. M. Openshaw, J. Kenneth Baillie, ISARIC4C Investigators, Stevan R. Emmett, Paul Digard, David A. Matthews, Lance Turtle, Alistair C. Darby, Andrew D. Davidson, Miles W. Carroll, Julian A. Hiscox

Publikováno v: Genome Biology, Vol 24, Iss 1, Pp 1-23 (2023)

Abstract Background The mutational landscape of SARS-CoV-2 varies at the dominant viral genome sequence and minor genomic variant population. During the COVID-19 pandemic, an early substitution in the genome was the D614G change in the spike protein,

Externí odkaz: https://doaj.org/article/b3d75d58548e46f4b79481a60a9b08d2

The Growth of Eimeria tenella: Characterization and Application of Quantitative Methods to Assess Sporozoite Invasion and Endogenous Development in Cell Culture

Autor: Virginia Marugan-Hernandez, Georgia Jeremiah, Kelsilandia Aguiar-Martins, Alana Burrell, Sue Vaughan, Dong Xia, Nadine Randle, Fiona Tomley

Publikováno v: Frontiers in Cellular and Infection Microbiology, Vol 10 (2020)

In vitro development of the complete life cycle of Eimeria species has been achieved in primary cultures of avian epithelial cells with low efficiency. The use of immortalized cell lines simplifies procedures but only allows partial development throu

Externí odkaz: https://doaj.org/article/5cae037101134aa9b8fb264a608e7e25

Amplicon-Based Detection and Sequencing of SARS-CoV-2 in Nasopharyngeal Swabs from Patients With COVID-19 and Identification of Deletions in the Viral Genome That Encode Proteins Involved in Interferon Antagonism

Autor: Shona C. Moore, Rebekah Penrice-Randal, Muhannad Alruwaili, Nadine Randle, Stuart Armstrong, Catherine Hartley, Sam Haldenby, Xiaofeng Dong, Abdulrahman Alrezaihi, Mai Almsaud, Eleanor Bentley, Jordan Clark, Isabel García-Dorival, Paul Gilmore, Ximeng Han, Benjamin Jones, Lisa Luu, Parul Sharma, Ghada Shawli, Yani Sun, Qin Zhao, Steven T. Pullan, Daniel P. Carter, Kevin Bewley, Jake Dunning, En-min Zhou, Tom Solomon, Michael Beadsworth, James Cruise, Derrick W. Crook, David A. Matthews, Andrew D. Davidson, Zana Mahmood, Waleed Aljabr, Julian Druce, Richard Vipond, Lisa Ng, Laurent Renia, Peter J. M. Openshaw, J. Kenneth Baillie, Miles W. Carroll, James Stewart, Alistair Darby, Malcolm Semple, Lance Turtle, Julian A. Hiscox

Publikováno v: Viruses, Vol 12, Iss 10, p 1164 (2020)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). Sequencing the viral genome as the outbreak progresses is important, particularly in the identification of emerging isolates w

Externí odkaz: https://doaj.org/article/5eae6c3757f94836aa348d618a86b709

Analysis of SARS-CoV-2 known and novel subgenomic mRNAs in cell culture, animal model, and clinical samples using LeTRS, a bioinformatic tool to identify unique sequence identifiers

Autor: Xiaofeng Dong, Rebekah Penrice-Randal, Hannah Goldswain, Tessa Prince, Nadine Randle, I'ah Donovan-Banfield, Francisco J Salguero, Julia Tree, Ecaterina Vamos, Charlotte Nelson, Jordan Clark, Yan Ryan, James P Stewart, Malcolm G Semple, J Kenneth Baillie, Peter J M Openshaw, Lance Turtle, David A Matthews, Miles W Carroll, Alistair C Darby, Julian A Hiscox

Publikováno v: Dong, X, Penrice-Randal, R, Goldswain, H, Prince, T, Randle, N, Donovan-Banfield, I, Salguero, F J, Tree, J, Vamos, E, Nelson, C, Clark, J, Ryan, Y, Stewart, J P, Semple, M G, Baillie, J K, Openshaw, P J M, Turtle, L, Matthews, D A, Carroll, M W, Darby, A C & Hiscox, J A 2022, ' Analysis of SARS-CoV-2 known and novel subgenomic mRNAs in cell culture, animal model, and clinical samples using LeTRS, a bioinformatic tool to identify unique sequence identifiers ', GigaScience, vol. 11, giac045, pp. 1-17 . https://doi.org/10.1093/gigascience/giac045

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has a complex strategy for the transcription of viral subgenomic mRNAs (sgmRNAs), which are targets for nucleic acid diagnostics. Each of these sgmRNAs has a unique 5′ sequence, the leade

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb36a7b5ad0e1d608fe30d77e4565510
https://www.pure.ed.ac.uk/ws/files/279437558/giac045.pdf

Identification and quantification of SARS-CoV-2 leader subgenomic mRNA gene junctions in nasopharyngeal samples shows phasic transcription in animal models of COVID-19 and dysregulation at later time points that can also be identified in humans

Autor: Javier Salguero, Charlotte Nelson, Peter J. M. Openshaw, Isaric C Investigators, David A. Matthews, Miles W. Carroll, Hannah Goldswain, Tessa Prince, Rebekah Penrice-Randal, Malcolm G Semple, Alistair C. Darby, Xiaofeng Dong, James P. Stewart, Julian A. Hiscox, Julia A. Tree, Ecaterina Vamos, Lance Turtle, Kenneth Baillie, Nadine Randle

IntroductionSARS-CoV-2 has a complex strategy for the transcription of viral subgenomic mRNAs (sgmRNAs), which are targets for nucleic acid diagnostics. Each of these sgRNAs has a unique 5’ sequence, the leader-transcriptional regulatory sequence g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cb5ccb8ffbc69b2eb43b23a93ffe8d42
https://doi.org/10.1101/2021.03.03.433753