Effects of Rationally Designed Physico-Chemical Variants of the Peptide PuroA on Biocidal Activity towards Bacterial and Mammalian Cells

Autor: Nadin Shagaghi, Enzo A. Palombo, Marie-Isabel Aguilar, Mrinal Bhave, Andrew H. A. Clayton, Tzong-Hsien Lee

Publikováno v: International Journal of Molecular Sciences
Volume 21
Issue 22
International Journal of Molecular Sciences, Vol 21, Iss 8624, p 8624 (2020)

Antimicrobial peptides (AMPs) often exhibit wide-spectrum activities and are considered ideal candidates for effectively controlling persistent and multidrug-resistant wound infections. PuroA, a synthetic peptide based on the tryptophan (Trp)-rich do

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31ed4b82112c2714aa6f7554227b1cb0

Using fluorescence lifetime dequenching to estimate the average quinary stoichiometry of proteins in living cells

Autor: Andrew H. A. Clayton, Nguyen Tran, Nadin Shagaghi

Publikováno v: Methods and applications in fluorescence. 8(1)

Biological proteins are understood in terms of five structural levels-primary, secondary, tertiary, quaternary and quinary. The quinary structure is defined as the set of macromolecular interactions that are transient in vivo. This includes non-coval

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19126d446266904ad1a462bbc0346cab
https://pubmed.ncbi.nlm.nih.gov/31622968

Selective Cytotoxicity of a Novel Trp‐Rich Peptide against Lung Tumor Spheroids Encapsulated inside a 3D Microfluidic Device

Autor: Huseyin Sumer, Nandini Dhiman, Peter Kingshott, Nadin Shagaghi, Subha Narayan Rath, Mrinal Bhave

Publikováno v: Advanced Biosystems. 4:1900285

There is a globally rising healthcare need to develop new anticancer therapies as well as to test them on biologically relevant in vitro cancer models instead of overly simplistic 2D models. To address both these needs, a 3D lung cancer spheroid mode

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6fc4e4682a4ace649b8e79a6e472e26
https://doi.org/10.1002/adbi.201900285

Revealing the sequence of interactions of PuroA peptide with Candida albicans cells by live-cell imaging

Autor: Mrinal Bhave, Nadin Shagaghi, Andrew H. A. Clayton, Enzo A. Palombo

Publikováno v: Scientific Reports

To determine the mechanism(s) of action of antimicrobial peptides (AMPs) it is desirable to provide details of their interaction kinetics with cellular, sub-cellular and molecular targets. The synthetic peptide, PuroA, displays potent antimicrobial a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bcfc1eef71a53781a1695164dcbd3eb
https://doi.org/10.1038/srep43542

Archetypal tryptophan-rich antimicrobial peptides: properties and applications

Autor: Enzo A. Palombo, Mrinal Bhave, Nadin Shagaghi, Andrew H. A. Clayton

Publikováno v: World Journal of Microbiology and Biotechnology. 32

Drug-resistant microorganisms ('superbugs') present a serious challenge to the success of antimicrobial treatments. Subsequently, there is a crucial need for novel bio-control agents. Many antimicrobial peptides (AMPs) show a broad-spectrum activity

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cb99b49ede330200f24d7501ca52f1a
https://doi.org/10.1007/s11274-015-1986-z