Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Nadia Taouatas"'
Autor:
Tokameh Mahmoudi, Sylvia F Boj, Pantelis Hatzis, Vivian S W Li, Nadia Taouatas, Robert G J Vries, Hans Teunissen, Harry Begthel, Jeroen Korving, Shabaz Mohammed, Albert J R Heck, Hans Clevers
Publikováno v:
PLoS Biology, Vol 15, Iss 2, p e1002596 (2017)
[This corrects the article DOI: 10.1371/journal.pbio.1000539.].
Externí odkaz:
https://doaj.org/article/62c1db93ed6448449dcd4f427ae3ddea
Autor:
Tokameh Mahmoudi, Sylvia F Boj, Pantelis Hatzis, Vivian S W Li, Nadia Taouatas, Robert G J Vries, Hans Teunissen, Harry Begthel, Jeroen Korving, Shabaz Mohammed, Albert J R Heck, Hans Clevers
Publikováno v:
PLoS Biology, Vol 8, Iss 11, p e1000539 (2010)
Wnt signaling maintains the undifferentiated state of intestinal crypt progenitor cells by inducing the formation of nuclear TCF4/β-catenin complexes. In colorectal cancer, activating mutations in Wnt pathway components cause inappropriate activatio
Externí odkaz:
https://doaj.org/article/645b939842eb4f87822b8f0fead8cb25
Autor:
Robert G.J. Vries, Albert J. R. Heck, Pantelis Hatzis, Tokameh Mahmoudi, Nadia Taouatas, Vivian S. W. Li, Hans Clevers, Hans Teunissen, Sylvia F. Boj, Harry Begthel, Jeroen Korving, Shabaz Mohammed
Publikováno v:
PLoS Biology, 8(11). Public Library of Science
PLoS Biology
PLoS Biology, Vol 8, Iss 11, p e1000539 (2010)
PLoS Biology
PLoS Biology, Vol 8, Iss 11, p e1000539 (2010)
The leukemia-associated Mllt10/Af10 and its partner the histone methyltransferase Dot1l are identified as Tcf4/β-catenin co-activators and shown to be essential for Wnt-driven endogenous gene expression, intestinal development and homeostasis.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf3e12c4305daee57f3a10d4a6859fdc
https://doi.org/10.1371/journal.pbio.1000539
https://doi.org/10.1371/journal.pbio.1000539
Autor:
Nadia Taouatas, Albert J. R. Heck, A. F. Maarten Altelaar, Andreas O. Helbig, Shabaz Mohammed, Madalina M. Drugan
In proteomics multi-dimensional fractionation techniques are widely used to reduce the complexity of peptide mixtures subjected to mass spectrometric analysis. Here, we describe the sequential use of strong cation exchange and reversed phase liquid c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76236eb14ec87a15ebbc3de9cdc43d9d
https://ora.ox.ac.uk/objects/uuid:cc051692-defb-4245-942f-1658483d273f
https://ora.ox.ac.uk/objects/uuid:cc051692-defb-4245-942f-1658483d273f
Publikováno v:
Nature Methods, 5(5), 405-407. Nature Publishing Group
We introduce a method for sequencing peptides by mass spectrometry using a metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). When analyzed by electron transfer dissociation (ETD)-based mass spectrometric sequencing, Lys-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3efe65570f5b026dd199ccc0669fcbfe
https://doi.org/10.1038/nmeth.1204
https://doi.org/10.1038/nmeth.1204
Autor:
Albert J. R. Heck, A. F. Maarten Altelaar, Paul J. Boersema, Joost W. Gouw, Darryl J. Pappin, Philip L. Ross, Nadia Taouatas, Shabaz Mohammed
In this work, we explore the potential of the metalloendopeptidase Lys-N for MALDI-MS/MS proteomics applications. Initially we digested a HEK293 cellular lysate with Lys-N and, for comparison, in parallel with the protease Lys-C. The resulting peptid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8726be8a5ff838821d71ac747a1c47ed
https://doi.org/10.1074/mcp.m800249-mcp200
https://doi.org/10.1074/mcp.m800249-mcp200
Trypsin, the most widely used enzyme in proteomics, has a few caveats as it does not perform well under certain harsh sample handling conditions and creates relatively short peptides less amenable to, for instance, electron transfer dissociation. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::497b280e402cafe6c08fd7804c049442
https://doi.org/10.1021/pr100341e
https://doi.org/10.1021/pr100341e
Autor:
Bas van Breukelen, Madalina M. Drugan, Albert J. R. Heck, Nadia Taouatas, Shabaz Mohammed, Alexandros Georgiou
Publikováno v:
PROTEOMICS. 10:1196-1201
Recently, we introduced a novel proteomics method employing a metalloendopeptidase with Lys-N specificity to produce proteolytic peptides. Fragmentation spectra generated by electron transfer dissociation, for a large proportion of the Lys-N proteoly
Autor:
Vivian S. W. Li, Hans Clevers, Shabaz Mohammed, Robert G.J. Vries, Tokameh Mahmoudi, Ser Sue Ng, Albert J. R. Heck, Nadia Taouatas
Publikováno v:
EMBO Journal, 28(21), 3329-3340. Nature Publishing Group
Wnt signalling maintains the undifferentiated state of intestinal crypt/progenitor cells through the TCF4/beta-catenin-activating transcriptional complex. In colorectal cancer, activating mutations in Wnt pathway components lead to inappropriate acti
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 753
The current advances in mass spectrometry technology have led to the possibility of analyzing more complex biological samples such as entire proteomes. Here, we describe a new and powerful methodology that combines the use of the metalloendopeptidase