Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Nadia Amrani"'
Autor:
Lin Zhao, Sabrina R. T. Koseki, Rachel A. Silverstein, Nadia Amrani, Christina Peng, Christian Kramme, Natasha Savic, Martin Pacesa, Tomás C. Rodríguez, Teodora Stan, Emma Tysinger, Lauren Hong, Vivian Yudistyra, Manvitha R. Ponnapati, Joseph M. Jacobson, George M. Church, Noah Jakimo, Ray Truant, Martin Jinek, Benjamin P. Kleinstiver, Erik J. Sontheimer, Pranam Chatterjee
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-8 (2023)
Abstract CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting doma
Externí odkaz:
https://doaj.org/article/c6eaa1afc2b34657a91e037a58ba1052
Autor:
Emmanouela Tsagkaraki, Sarah M. Nicoloro, Tiffany DeSouza, Javier Solivan-Rivera, Anand Desai, Lawrence M. Lifshitz, Yuefei Shen, Mark Kelly, Adilson Guilherme, Felipe Henriques, Nadia Amrani, Raed Ibraheim, Tomas C. Rodriguez, Kevin Luk, Stacy Maitland, Randall H. Friedline, Lauren Tauer, Xiaodi Hu, Jason K. Kim, Scot A. Wolfe, Erik J. Sontheimer, Silvia Corvera, Michael P. Czech
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
Worldwide pandemics of obesity and diabetes prompt an urgent need for new approaches to their prevention and cure. Here the authors present a CRISPR-based strategy that enhances the therapeutic potential of human adipocytes when implanted in obese mi
Externí odkaz:
https://doaj.org/article/9db95209ec9c4296b7f96552abbf9db0
Autor:
Nadia Amrani, Xin D. Gao, Pengpeng Liu, Alireza Edraki, Aamir Mir, Raed Ibraheim, Ankit Gupta, Kanae E. Sasaki, Tong Wu, Paul D. Donohoue, Alexander H. Settle, Alexandra M. Lied, Kyle McGovern, Chris K. Fuller, Peter Cameron, Thomas G. Fazzio, Lihua Julie Zhu, Scot A. Wolfe, Erik J. Sontheimer
Publikováno v:
Genome Biology, Vol 19, Iss 1, Pp 1-25 (2018)
Abstract Background The development of CRISPR genome editing has transformed biomedical research. Most applications reported thus far rely upon the Cas9 protein from Streptococcus pyogenes SF370 (SpyCas9). With many RNA guides, wildtype SpyCas9 can i
Externí odkaz:
https://doaj.org/article/d001df012bc241759e81c0e2bc14037f
Autor:
Mehmet Fatih Bolukbasi, Pengpeng Liu, Kevin Luk, Samantha F. Kwok, Ankit Gupta, Nadia Amrani, Erik J. Sontheimer, Lihua Julie Zhu, Scot A. Wolfe
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
Therapeutic genome engineering relies on the development of reliable, robust and versatile tools. Here the authors develop Cas9-Cas9 chimeras with high target site activity that generate predictable deletions.
Externí odkaz:
https://doaj.org/article/463e5b127556499185f48ce65924481b
All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
Publikováno v:
Genome Biology, Vol 19, Iss 1, Pp 1-11 (2018)
Abstract Background Clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) have recently opened a new avenue for gene therapy. Cas9 nuclease guided by a single-guide RNA (sgRNA) has been extensively
Externí odkaz:
https://doaj.org/article/6ab1316dcca84075aa5b30d20fb4f776
Autor:
Jooyoung Lee, Aamir Mir, Alireza Edraki, Bianca Garcia, Nadia Amrani, Hannah E. Lou, Ildar Gainetdinov, April Pawluk, Raed Ibraheim, Xin D. Gao, Pengpeng Liu, Alan R. Davidson, Karen L. Maxwell, Erik J. Sontheimer
Publikováno v:
mBio, Vol 9, Iss 6 (2018)
ABSTRACT In their natural settings, CRISPR-Cas systems play crucial roles in bacterial and archaeal adaptive immunity to protect against phages and other mobile genetic elements, and they are also widely used as genome engineering technologies. Previ
Externí odkaz:
https://doaj.org/article/235e55b82aeb4666b874a7f3a5f87dce
Autor:
Mehmet Fatih Bolukbasi, Pengpeng Liu, Kevin Luk, Samantha F. Kwok, Ankit Gupta, Nadia Amrani, Erik J. Sontheimer, Lihua Julie Zhu, Scot A. Wolfe
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-1 (2018)
The original version of this Article contained errors in the author affiliations. Mehmet Fatih Bolukbasi was incorrectly associated with Bluebird Bio., Cambridge, MA, USA and Ankit Gupta was incorrectly associated with Exonics Therapeutics, Watertown
Externí odkaz:
https://doaj.org/article/72f5635a3ba44658adb454734a993620
Autor:
Sabrina Koseki, Lauren Hong, Vivian Yudistyra, Teodora Stan, Emma Tysinger, Rachel Silverstein, Christian Kramme, Nadia Amrani, Natasha Savic, Martin Pacesa, Tomás Rodriguez, Manvitha Ponnapati, Joseph Jacobson, George Church, Ray Truant, Martin Jinek, Benjamin Kleinstiver, Erik Sontheimer, Pranam Chatterjee
CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting domain of SpR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ccc773ed8423a1eacd9b56425b4f34ee
https://doi.org/10.21203/rs.3.rs-2625838/v1
https://doi.org/10.21203/rs.3.rs-2625838/v1
Autor:
Michael P. Czech, Tomás Rodríguez, Randall H. Friedline, Silvia Corvera, Raed Ibraheim, Lauren A. Tauer, Jason K. Kim, Kevin Luk, Sarah M. Nicoloro, Tiffany DeSouza, Lawrence M. Lifshitz, Anand Desai, Emmanouela Tsagkaraki, Xiaodi Hu, Felipe Henriques, Yuefei Shen, Nadia Amrani, Stacy Maitland, Mark J. S. Kelly, Scot A. Wolfe, Erik J. Sontheimer, Adilson L. Guilherme, Javier Solivan-Rivera
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
Nature Communications
Nature Communications
Obesity and type 2 diabetes are associated with disturbances in insulin-regulated glucose and lipid fluxes and severe comorbidities including cardiovascular disease and steatohepatitis. Whole body metabolism is regulated by lipid-storing white adipoc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcb551651850ab32eb22e3befd059530
https://doi.org/10.1038/s41467-021-27190-y
https://doi.org/10.1038/s41467-021-27190-y
Autor:
Tomás C, Rodríguez, Sina, Dadafarin, Henry E, Pratt, PengPeng, Liu, Nadia, Amrani, Lihua Julie, Zhu
Publikováno v:
Progress in molecular biology and translational science. 181
The clustered, regularly interspersed, short palindromic repeats (CRISPR) technology is revolutionizing biological studies and holds tremendous promise for treating human diseases. However, a significant limitation of this technology is that modifica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8aa5aaced2f9230a1903fcbff7506b53
https://pubmed.ncbi.nlm.nih.gov/34127199
https://pubmed.ncbi.nlm.nih.gov/34127199