Removal of nonspecific binding proteins is required in co-immunoprecipitation with nuclear proteins

Autor: Xi Zeng, Wei-Hong Zeng, Jun Zhou, Xiao-Min Liu, Guo Huang, Hong Zhu, Shuai Xiao, Ying Zeng, Deliang Cao

Publikováno v: BioTechniques, Vol 73, Iss 6, Pp 289-296 (2022)

Whether protein samples should be pretreated to remove nonspecific binding proteins in co-immunoprecipitation (CO-IP) is controversial. In this work, nonspecific binding of proteins to agarose beads was found to be greater than that to magnetic beads

Externí odkaz: https://doaj.org/article/1ad96a03ff364d0d87e58f8bfc8f8529

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Evaluation and Refinement of Sample Preparation Methods for Extracellular Matrix Proteome Coverage

Autor: Monika Dzieciatkowska, Lauren R. Schmitt, Toin H. van Kuppevelt, Willeke F. Daamen, Mark Maslanka, Ryan C. Hill, Maxwell C. McCabe, Kirk C. Hansen, Danique J. Hof

Publikováno v: Molecular & Cellular Proteomics : MCP
Molecular & Cellular Proteomics, 20

The extracellular matrix is a key component of tissues, yet it is underrepresented in proteomic datasets. Identification and evaluation of proteins in the extracellular matrix (ECM) has proved challenging due to the insolubility of many ECM proteins

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b2083f5875a9c4968507a0ff8208d5d
http://europepmc.org/articles/PMC8188056

Oxygen and Conformation Dependent Protein Oxidation and Aggregation by Porphyrins in Hepatocytes and Light-Exposed CellsSummary

Autor: Nicolai Lehnert, Dhiman Maitra, Stephen W. Ragsdale, Laure Rittié, M. Bishr Omary, Alexey I. Nesvizhskii, Haoming Zhang, Matthew W. Wolf, Harald Herrmann, Venkatesha Basrur, Rani Richardson, Yoichi Osawa, Eric L. Carter

Publikováno v: Cellular and Molecular Gastroenterology and Hepatology, Vol 8, Iss 4, Pp 659-682.e1 (2019)
Cellular and Molecular Gastroenterology and Hepatology

Background & Aims Porphyrias are caused by porphyrin accumulation resulting from defects in the heme biosynthetic pathway that typically lead to photosensitivity and possible end-stage liver disease with an increased risk of hepatocellular carcinoma.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0592cf3cff3ae349fa52696e431bb166
http://www.sciencedirect.com/science/article/pii/S2352345X19300724

TMEM16A Ca

Autor: Qinghua, Wang, Lichuan, Bai, Shuya, Luo, Tianyu, Wang, Fan, Yang, Jialin, Xia, Hui, Wang, Ke, Ma, Mei, Liu, Shuwei, Wu, Huijie, Wang, Shibin, Guo, Xiaohong, Sun, Qinghuan, Xiao

Publikováno v: Journal of Advanced Research

Graphical abstract A positive activation loop between TMEM16A and the IP3R/Ca2+/NFκB/IL-6 pathway is important for Ca2+ elevation, NFκB activation and IL-6 release, and thus cooperatively promotes the pathogenesis of AP.
TMEM16A Ca2+-activated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a2a3e77ba7ea409a56ae2bf0b475cc7c
https://pubmed.ncbi.nlm.nih.gov/32071789