Unambiguous Tracking of Protein Phosphorylation by Fast High‐Resolution FOSY NMR**

Autor: Wolfgang Bermel, Eduard V. Bocharov, Vladislav Yu. Orekhov, Björn M. Burmann, Irena Matečko-Burmann, Panagiota S. Georgoulia, Dmitry M. Lesovoy, Tammo Diercks

Publikováno v: Angewandte Chemie (International Ed. in English)

Dysregulation of post‐translational modifications (PTMs) like phosphorylation is often involved in disease. NMR may elucidate exact loci and time courses of PTMs at atomic resolution and near‐physiological conditions but requires signal assignmen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dc4cde3dca26f335f48d92a6fda6011
https://doi.org/10.1002/ange.202102758

NMR Spectroscopic Characterization of the C‐Mannose Conformation in a Thrombospondin Repeat Using a Selective Labeling Approach

Autor: Hendrik R. A. Jonker, Birgit Tiemann, Hans Bakker, Aleksandra Shcherbakova, Krishna Saxena, Harald Schwalbe

Publikováno v: Angewandte Chemie (International Ed. in English)

Despite the great interest in glycoproteins, structural information reporting on conformation and dynamics of the sugar moieties are limited. We present a new biochemical method to express proteins with glycans that are selectively labeled with NMR�

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39c59af00da3de409dd111d0fea04af5
https://doi.org/10.1002/ange.202009489

Disease‐Associated Tau Phosphorylation Hinders Tubulin Assembly within Tau Condensates

Autor: David Flores, Markus Zweckstetter, Adriana Savastano, Eckhard Mandelkow, Harindranath Kadavath, Jacek Biernat

Publikováno v: Angewandte Chemie International Edition
Angewandte Chemie (International Ed. in English)
Angewandte Chemie. International Edition, 60 (2)
Angewandte Chemie / International edition 60(2), 726-730 (2021). doi:10.1002/anie.202011157

Cellular condensation of intrinsically disordered proteins (IDPs) through liquid–liquid phase separation (LLPS) allows dynamic compartmentalization and regulation of biological processes. The IDP tau, which promotes the assembly of microtubules and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e36ff0e09e5d7eb79385f20ffb36105f

Single‐Scan Selective Excitation of Individual NMR Signals in Overlapping Multiplets

Autor: Peter Kiraly, Nicolas Kern, Mateusz P. Plesniak, Mathias Nilsson, David J. Procter, Gareth A. Morris, Ralph W. Adams

Publikováno v: Angewandte Chemie International Edition
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2021, 60 (2), pp.666-669. ⟨10.1002/anie.202011642⟩
Angewandte Chemie (International Ed. in English)

2D NMR is an immensely powerful structural tool but it is time‐consuming. Targeting individual chemical groups by selective excitation in a 1D experiment can give the information required far more quickly. A major problem, however, is that proton N

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62ee3acef552b64e93347d05507d445b
https://hal.archives-ouvertes.fr/hal-03425427/file/anie.202011642.pdf

PI by NMR: Probing CH-π Interactions in Protein-Ligand Complexes by NMR Spectroscopy

Autor: Sven Brüschweiler, Robert Konrat, Julia Schörghuber, Darryl B. McConnell, Gerd Bader, Andreas Beier, Bernhard Wolkerstorfer, Leonhard Geist, Dirk Kessler, Harald Engelhardt, Moriz Mayer, Gerald Platzer, Julian E. Fuchs, Roman J. Lichtenecker

Publikováno v: Angewandte Chemie (International Ed. in English)

While CH–π interactions with target proteins are crucial determinants for the affinity of arguably every drug molecule, no method exists to directly measure the strength of individual CH–π interactions in drug–protein complexes. Herein, we pr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82f4c4ecbf6441f842757fe25e95d829
https://pubmed.ncbi.nlm.nih.gov/32421895