Zobrazeno 1 - 10
of 247
pro vyhledávání: '"N.N. Ugarova"'
Publikováno v:
Moscow University Chemistry Bulletin. 75:374-381
The kinetics of the hydrolysis of ATP by apyrase A from the Solanum tuberosum potato is studied at a pH of 6.5 and 25°C by the bioluminescent method. KM = 33 μM and V = 0.37 μM/s are determined for ATP. It is shown that the hydrolysis of ATP proce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::866f377f32c7e650978ab0c985a92c6c
https://doi.org/10.3103/s0027131420060139
https://doi.org/10.3103/s0027131420060139
Publikováno v:
Moscow University Chemistry Bulletin. 75:186-194
Digitonin (monodesmoid saponin) forms complexes with cholesterol of cell membranes, causing the disintegration of the membrane structure, the formation of pores, and the release of intracellular components in the reaction medium. We use HEK293 cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::41982330075d8d12ea82c7002064a391
https://doi.org/10.3103/s0027131420030086
https://doi.org/10.3103/s0027131420030086
Autor:
Galina Novichkova, Mikhail A. Panteleev, Anastasia A. Ignatova, N.N. Ugarova, Sergey I. Obydennyi, Fazoil I. Ataullakhanov, Tatiana V. Varlamova, Elena O. Artemenko, Igor I. Kireev, Anastasia N. Sveshnikova, Anna Shcherbina, Stepan Gambaryan, Aleksey Maschan, G.Y. Lomakina
Publikováno v:
Haematologica
Wiskott-Aldrich syndrome (WAS) is associated with thrombocytopenia of unclear origin. We investigated real-time cytosolic calcium dynamics, mitochondrial membrane potential and phoszphatidylserine (PS) exposure in single fibrinogen-bound platelets us
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c77189bf45aeda5c04238a91c8852145
http://europepmc.org/articles/PMC7109739
http://europepmc.org/articles/PMC7109739
Autor:
N.N. Ugarova, G. Yu. Lomakina
Publikováno v:
Moscow University Chemistry Bulletin. 75:15-21
The distinctive features of firefly luciferase bioluminescence are complex changes in the shape of the spectra and λmax of bioluminescence with varying pH, temperature, and enzyme structure. An analysis of the published data and the authors’ own r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::085a80e2adf7b1fdb3b07160557b610e
https://doi.org/10.3103/s0027131420010113
https://doi.org/10.3103/s0027131420010113
Autor:
E.N. Efremenko, N.N. Ugarova, G.Y. Lomakina, O.V. Senko, N.A. Stepanov, O.V. Maslova, A.G. Aslanly, I.V. Lyagin
The collective monograph is devoted to demonstrating and discussing the possibilities of using a highly sensitive bioluminescent method for analyzing concentrations of adenosine triphosphate (ATP) in various objects and fields of research: in sanitat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::775d567e7d2d8727844cb8b21b6f6ee8
https://doi.org/10.36871/978-5-907497-77-1
https://doi.org/10.36871/978-5-907497-77-1
Publikováno v:
Moscow University Chemistry Bulletin. 74:191-197
The Bacille Calmette–Guerine (BCG) vaccine, which is based on a live strain of Mycobacterium bovis BCG is widely used for the immunoprophylaxis of tuberculosis. One of the BCG vaccine’s key parameters is its сell viability (specific activity: th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::222f35d67d856a81991accb463ec7397
https://doi.org/10.3103/s0027131419040084
https://doi.org/10.3103/s0027131419040084
Publikováno v:
Moscow University Chemistry Bulletin. 73:158-165
Cells and tissues are composed from atoms of chemical elements, some of which have two kinds of stable isotopes, magnetic and nonmagnetic ones. Not long ago, magnetic isotope effects (MIEs) have been discovered in experiments with cells enriched with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::71b6a955a50f881613f4267c7b1d489a
https://doi.org/10.3103/s0027131418040119
https://doi.org/10.3103/s0027131418040119
Autor:
D. V. Smirnova, N.N. Ugarova
Publikováno v:
Photochemistry and Photobiology. 93:436-447
Firefly luciferase is widely used in molecular biology and bioanalytical systems as a reporter molecule due to the high quantum yield of the bioluminescence, availability of stable mutant forms of the enzyme with prescribed spectral characteristics a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1feab2a0a7484954359aa687588d0f3
https://doi.org/10.1111/php.12656
https://doi.org/10.1111/php.12656
Autor:
Darya V Smirnova, N.N. Ugarova
Publikováno v:
Combinatorial Chemistry & High Throughput Screening. 18:946-951
Bioanalytical systems based on the Bioluminescence Resonance Energy Transfer (BRET) are widely used in fundamental biochemical studies, as well as for screening and analysis of biologically active compounds. The Renilla luciferase is the most often u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45be19b800611cd4fcc9e48210012229
https://doi.org/10.2174/1386207318666150917095731
https://doi.org/10.2174/1386207318666150917095731
Publikováno v:
Moscow University Chemistry Bulletin. 69:49-55
A method has been developed for synthesizing stable and highly active conjugates of firefly luciferase with antibodies using the heterobifunctional coupling agent N-succinimidyl 3-(2-pyridyldithio) propionate linking NH2 groups of antibodies with fre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b197454350a0aec0509494995e27c023
https://doi.org/10.3103/s0027131414020047
https://doi.org/10.3103/s0027131414020047