Zobrazeno 1 - 10
of 14
pro vyhledávání: '"N. Rao Thotakura"'
Autor:
Gattadahalli S. Seetharamaiah, Robert L. Peake, John S. Dallas, N. Rao Thotakura, Bellur S. Prabhakar, Sai A. Patibandla
Publikováno v:
Endocrinology. 138:1559-1566
We expressed the extracellular domain of the mouse TSH receptor (mET-gp) using the baculovirus expression system. The recombinant protein was identified as mET-gp by immunoblotting and N-terminal amino acid sequencing. Carbohydrate analysis of the re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8efa417c90bf7ca4c35aad3e4c203719
https://doi.org/10.1210/endo.138.4.5093
https://doi.org/10.1210/endo.138.4.5093
Autor:
L A Bishop, Peter R. Schofield, Mathis Grossmann, Mariusz W. Szkudlinski, Joseph E. Tropea, Bruce D. Weintraub, N. Rao Thotakura
Publikováno v:
Journal of Biological Chemistry. 270:29378-29385
We used a novel approach to study the role of the Asn-linked oligosaccharides for human thyrotropin (hTSH) activity. Mutagenesis of Asn (N) within individual glycosylation recognition sequences to Gln (Q) was combined with expression of wild type and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::820f75206cc8ff9619f3bd4237a649b0
https://doi.org/10.1074/jbc.270.49.29378
https://doi.org/10.1074/jbc.270.49.29378
Publikováno v:
Glycobiology. 4:525-533
Recombinant human thyrotrophin (rhTSH) contains oligosaccharides terminating in -galactose-sialic acid, and had lower metabolic clearance and higher in vivo bioactivity compared to pituitary hTSH, which has oligosaccharides terminating predominantly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3b4f6068a31ce3b96aaa3e88e51d57d
https://doi.org/10.1093/glycob/4.4.525
https://doi.org/10.1093/glycob/4.4.525
Publikováno v:
Biochemical and Biophysical Research Communications. 186:301-307
Insulin-like growth factor binding protein-6 is abundant in cerebrospinal fluid and has a marked preferential binding affinity for IGF-II over IGF-I. The present study demonstrates that IGFBP-6 is O-glycosylated but not N-glycosylated. Carbohydrate a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c032f1faeda6ce2ae127fe92221cf1ad
https://doi.org/10.1016/s0006-291x(05)80807-1
https://doi.org/10.1016/s0006-291x(05)80807-1
Publikováno v:
Molecular and Cellular Endocrinology. 78:25-32
Estrogen synthetase (aromatase) is a cytochrome P-450 enzyme system which converts androgens to estrogens. Although this enzyme has been purified and the cDNA-derived amino acid sequence elucidated, very little is known regarding post-translational m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3cb4f9c60f9071e2e6606427de45e74
https://doi.org/10.1016/0303-7207(91)90182-r
https://doi.org/10.1016/0303-7207(91)90182-r
Autor:
Diana L. Blithe, Bruce B. Reinhold, Vernon N. Reinhold, N. Rao Thotakura, Martin Nemansky, Curtis D. Lyons, Song Ye
Publikováno v:
The Journal of biological chemistry. 273(20)
Glycoprotein hormone alpha subunit, in its free form (free alpha), is a major placental product. Its glycosylation was found to change dramatically during the advancement of pregnancy. In this study, we have analyzed these glycosylation changes in fi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8bb0a456811aa4af0e8e7e5795c98cd
https://pubmed.ncbi.nlm.nih.gov/9575149
https://pubmed.ncbi.nlm.nih.gov/9575149
Autor:
Bruce D. Weintraub, Mariusz W. Szkudlinski, John Y K Lee, N. Rao Thotakura, Joyce East-Palmer
Publikováno v:
Thyroid : official journal of the American Thyroid Association. 5(1)
A new and simple in vivo bioassay suitable for routine testing of pituitary and recombinant TSH preparations was developed. Male Albino Swiss CF-1 mice were given T3 in their drinking water to suppress endogenous TSH. T3, 3.0 micrograms/mL, given to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2669927bbdf873da21751944e156d55
https://pubmed.ncbi.nlm.nih.gov/7787435
https://pubmed.ncbi.nlm.nih.gov/7787435
Autor:
Yoko Murata, Bruce D. Weintraub, Lata Joshi, Myung K. Kim, N. Rao Thotakura, Mariusz W. Szkudlinski, D L Bodenner
Publikováno v:
Glycoprotein Hormones ISBN: 9781461383888
Thyroid stimulating hormone (TSH) is an anterior pituitary hormone that stimulates thyroid hormone production (1). It is composed of two subunits: the alpha subunit, which is in common with the gonadotropins, luteinizing hormone, and follicle stimula
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1010b2f7f8625ec3458e79c8256ad397
https://doi.org/10.1007/978-1-4613-8386-4_8
https://doi.org/10.1007/978-1-4613-8386-4_8
Autor:
Lisa G. Bates, Bruce M. Pratt, Rajesh K. Desai, Bruce D. Weintraub, N. Rao Thotakura, Edward S. Cole
Publikováno v:
Endocrinology. 128(1)
The presence and specific structures of the oligosaccharides on TSH have been shown to be important for its production and bioactivity. Since the carbohydrate structure of a protein reflects the glycosylation apparatus of the host cells in which the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7627c0af8cc28d7d3525593f62ea161c
https://pubmed.ncbi.nlm.nih.gov/1846103
https://pubmed.ncbi.nlm.nih.gov/1846103
Autor:
Bethel Stannard, Catherine Ronin, N. Rao Thotakura, Peter W. Gyves, Neil Gesundheit, Bruce D. Weintraub
Publikováno v:
Biochemical and biophysical research communications. 165(2)
Summary We examined the effect of various inhibitors of oligosaccharide processing on the content and secretion of newly synthesized thyroid-stimulating hormone (TSH) from dispersed hypothyroid rodent pituitary cells. 1-deoxynojirimycin and N-methyl-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b4431ceb0c357e8a9e9d6e485a5a422
https://pubmed.ncbi.nlm.nih.gov/2532008
https://pubmed.ncbi.nlm.nih.gov/2532008