Zobrazeno 1 - 10
of 83
pro vyhledávání: '"N. M. Lehnert"'
Publikováno v:
Toxicology. 160:119-127
Inhalation of particulate beryllium (Be) and its compounds causes chronic Be disease (CBD) in a relatively small subset ( approximately 1-6%) of exposed individuals. Hallmarks of this pulmonary disease include increases in several cell types, includi
Publikováno v:
Cytometry. 37:275-283
Background: The primary source of interference in immunofluorescence measurements by flow cytometry is background autofluorescence. Methods: Using human lung fibroblasts (HLFs) as an autofluorescent cell model, unfixed HLFs and HLFs fixed in methanol
Autor:
G. M. Jones, Larry A. Sklar, John A. Steinkamp, N. M. Lehnert, Bruce E. Lehnert, Chiranjit Deka
Publikováno v:
Cytometry. 25:271-279
Fluorescent antibodies are often used to measure the number of receptor sites on cells. The quantitative estimate of the number of receptor sites using this procedure assumes that the fluorescence intensity on a cell is proportional to the number of
Autor:
D. C. Archuleta, Bruce E. Lehnert, D. M. Stavert, N. M. Lehnert, Yolanda E. Valdez, Alina Deshpande
Publikováno v:
Inhalation Toxicology. 8:65-80
Several lines of circumstantial evidence suggest that an elevated production of tumor necrosis factor-α (TNF-α) by lung macrophages may play a role in the development of phosgene-induced pulmonary edema. The present study was undertaken to investig
Autor:
T Ellis, W S Session, D. M. Stavert, N. M. Lehnert, Bruce E. Lehnert, Lawrence R. Gurley, D. C. Archuleta
Publikováno v:
Toxicology. 89:239-277
Human inhalation exposures to relatively high mass concentrations of the oxidant gas nitrogen dioxide (NO2) can result in a variety of pulmonary disorders, including life-threatening pulmonary edema, pneumonia, and bronchiolitis obliterans. Inasmuch
Autor:
Yolanda E. Valdez, N. M. Lehnert, Bruce E. Lehnert, G. C. Saunders, R.J. Sebring, J. A. Steinkamp
Publikováno v:
American Journal of Respiratory Cell and Molecular Biology. 3:377-391
Airway intra-luminal macrophages (AI-LM) are a little-studied subpopulation of pulmonary macrophages that are located on the surfaces of the conducting airways of the lower respiratory tract. In this study, we: (1) developed a flow cytometric approac
Publikováno v:
Biomedical Optical Spectroscopy and Diagnostics.
We demonstrate a high speed method to analyze the impact of self-quenching on fluorescence lifetimes in individual cells by phase-sensitive flow cytometry, using a model system consisting of FITC (fluorescein isothiocyanate) labeled antimouse Thy 1.2
Autor:
Goutam Gupta, N. M. Lehnert, Bruce E. Lehnert, David L. Allen, Beth Allen, Patrick R. Shiflett, Michael Chen, P. Catasti
Publikováno v:
Biochemistry. 40(14)
Key surface proteins of pathogens and their toxins bind to the host cell receptors in a manner that is quite different from the way the natural ligands bind to the same receptors and direct normal cellular responses. Here we describe a novel strategy
Publikováno v:
Cytometry. 37(4)
The primary source of interference in immunofluorescence measurements by flow cytometry is background autofluorescence.Using human lung fibroblasts (HLFs) as an autofluorescent cell model, unfixed HLFs and HLFs fixed in methanol, ethanol, formaldehyd
Publikováno v:
Journal of immunological methods. 226(1-2)
We report a flow cytometric fluorescence lifetime-based method to discriminate damaged/dead from viable cells in immunofluorescently labeled populations using propidium iodide as a dye-exclusion viability probe. Fluorescence signals from propidium io