Zobrazeno 1 - 10
of 53
pro vyhledávání: '"N. K. Wills"'
Publikováno v:
American Journal of Physiology-Cell Physiology. 286:C79-C89
The human hereditary disorder Dent's disease is linked to loss-of-function mutations of the chloride channel ClC-5. Many of these mutations involve insertion of premature stop codons, resulting in truncation of the protein. We determined whether the
Autor:
Brian G. Kennedy, G. F. Jin, Tianxiang Weng, Nancy J. Mangini, N. K. Wills, Alan S.L. Yu, B. F. Godley
Publikováno v:
American Journal of Physiology-Cell Physiology. 283:C839-C849
Retinal pigment epithelium (RPE) possesses regulated chloride channels that are crucial for transepithelial fluid and ion transport. At present, little is known about the molecular nature of chloride channels in human adult RPE (haRPE) or the effects
Publikováno v:
American Journal of Physiology-Cell Physiology. 280:C1511-C1520
Genetic mutations of the Cl−channel ClC-5 cause Dent's disease in humans. We recently cloned an amphibian ortholog of Xenopus ClC-5 (xClC-5) from the A6 cell line. We now compare the properties and regulation of ClC-5 currents expressed in mammalia
Publikováno v:
Journal of Membrane Biology. 160:27-38
Previous work from this laboratory has shown that apical membrane sodium channel activity is stimulated by serosal hyposmotic solutions (Wills, MillinoffCrowe, 1991). In the present study, we determined whether this stimulation of sodium transport is
Publikováno v:
American Journal of Physiology-Cell Physiology. 273:C1176-C1185
Cl−channels are important for ion transport and cell volume regulation in A6 renal cells. In the present study, we used reverse transcriptase (RT)-polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) to identify proteins homo
Publikováno v:
Toxicology letters. 198(1)
Cadmium is reported to accumulate in human eye tissues suggesting its implication in diverse ocular pathology. Using an in vitro cell culture model we investigated the effects of cadmium on human lens epithelial cells (HLECs) (HLE-B3). We observed ca
Autor:
Frederik J.G.M. van Kuijk, N. K. Wills, Kota V. Ramana, Nilesh M. Kalariya, Satish K. Srivastava
Publikováno v:
Experimental eye research. 89(4)
Cadmium (Cd), released from cigarette smoke and metal industrial activities, is known to accumulate in human body organs including retina and is particularly higher in retinal tissues of age-related macular degeneration (AMD) eyes compared to non-AMD
Autor:
F. J. G. M. van Kuijk, V. M. Sadagopa Ramanujam, A. Husain, J.R. Lewis, S. Haji Abdollahi, N. K. Wills, Nilesh M. Kalariya
Publikováno v:
Experimental eye research. 89(1)
Cadmium is a naturally occurring, highly toxic, metallic element. It pollutes the environment as a result of industrial activity and accumulates in human tissues with a long biological half-life. Cadmium content has been demonstrated to increase in h
Publikováno v:
Experimental eye research. 87(2)
The essential metals copper and zinc play vital roles in retinal cell survival and are crucial for the normal functioning of antioxidant enzymes. Retinal zinc deficiencies and decreased cellular antioxidative capacity have been linked to human retina
Publikováno v:
American Journal of Physiology-Cell Physiology. 259:C869-C882
Urinary kallikreins are proteolytic enzymes known to be secreted by distal nephron tubules. In this study, we demonstrate (using the chromogenic tripeptide substrate S 2266) that the renal cell line A6 from Xenopus laevis secretes a kallikrein-like e