Zobrazeno 1 - 8
of 8
pro vyhledávání: '"N. K. Pryer"'
Publikováno v:
The Journal of Cell Biology
Although the mechanism of microtubule dynamic instability is thought to involve the hydrolysis of tubulin-bound GTP, the mechanism of GTP hydrolysis and the basis of microtubule stability are controversial. Video microscopy of individual microtubules
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bffda34f3f8e924f04f4b92fd68087c
https://doi.org/10.1083/jcb.114.1.73
https://doi.org/10.1083/jcb.114.1.73
Publikováno v:
Journal of Experimental Zoology. 253:163-176
We used silicone rubber substrata and fluorescent staining of cytoskeletal components to study the mechanisms by which electrical voltage gradients cause reorientation of embryonic chick fibroblasts in tissue culture. No evidence was found for a dire
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bab7bdf75ecded44fb4cda11fdd99b0
https://doi.org/10.1002/jez.1402530206
https://doi.org/10.1002/jez.1402530206
Autor:
Michael F. Soboeiro, Victoria Petrie Skeen, R. A. Walker, Brenda D. Bourns, N. K. Pryer, Edward D. Salmon
Publikováno v:
Journal of cell science. 103
We used video assays to study the dynamic instability behavior of individual microtubules assembled in vitro with purified tau, purified MAP2 or a preparation of unfractionated heat-stable MAPs. Axoneme-nucleated microtubules were assembled from pure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29965010279a6ad26431994055be28d8
https://pubmed.ncbi.nlm.nih.gov/1487507
https://pubmed.ncbi.nlm.nih.gov/1487507
Publikováno v:
The Journal of Cell Biology
Individual microtubule dynamics were observed in real time in primary cultures of newt lung epithelium using video-enhanced differential interference contrast microscopy and digital image processing. The linear filaments observed in cells corresponde
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d7c55838c40a7fff3dc85e77d32f433
http://europepmc.org/articles/PMC2115680
http://europepmc.org/articles/PMC2115680
Publikováno v:
BioEssays. 7:149-154
Recent evidence shows that dynamic instability is the dominant mechanism for the assembly of pure tubulin in vitro and for the great majority of microtubules in the mitotic spindle and the interphase cytoplasmic microtubule complex. The basic concept
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11982542edfca56440338a33e11ab990
https://doi.org/10.1002/bies.950070403
https://doi.org/10.1002/bies.950070403
Autor:
E T O'Brien, R. A. Walker, N. K. Pryer, Harold P. Erickson, W. A. Voter, M F Soboeiro, Edward D. Salmon
Publikováno v:
The Journal of Cell Biology
We have developed video microscopy methods to visualize the assembly and disassembly of individual microtubules at 33-ms intervals. Porcine brain tubulin, free of microtubule-associated proteins, was assembled onto axoneme fragments at 37 degrees C,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e558e76e1a2b5f3eb78f8c69837a38db
http://europepmc.org/articles/PMC2115242
http://europepmc.org/articles/PMC2115242
Publikováno v:
Cell motility and the cytoskeleton. 6(6)
In this report, we describe an in vitro system for analyzing microtubule-based movements in supernatants of sea urchin egg and embryo homogenates. Using video enhanced DIC microscopy, we have observed bidirectional saltatory particle movements on nat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a648c3a886c9a515ebc6e4e171dfc9fd
https://pubmed.ncbi.nlm.nih.gov/2879641
https://pubmed.ncbi.nlm.nih.gov/2879641
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 45:636-637
Microtubules (Mts) of the interphase cytoplasmic Mt complex (CMTC) and the mitotic spindle are dynamic polymers assembled from a cellular pool of tubulin by end-dependent association-dissociation reactions (1,2). Mts have an intrinsic structural pola
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7832ba27d4c68e74dda2b19a8ff1fa71
https://doi.org/10.1017/s042482010012761x
https://doi.org/10.1017/s042482010012761x