Zobrazeno 1 - 10
of 10
pro vyhledávání: '"N. I. Minaeva"'
Autor:
N. I. Minaeva
Publikováno v:
Archaeology and Early History of Ukraine. 42:193-202
This article considers the impact of changes in the natural environment on historical processes that took place in the steppe and forest-steppe zones of Ukraine in the XI—V centuries BC. It is determined that one of the main reasons for the emergen
Publikováno v:
Journal of Machinery Manufacture and Reliability. 50:41-45
The pattern of the interaction between powder particles and metal vapors is developed. To ensure a guaranteed supply of PAs to the heated region with the use of laser irradiation, a model for theoretically analyzing the motion paths of PA particles c
Publikováno v:
Russian Journal of Genetics. 46:308-314
MG1655 of Escherichia coli K-12 is frequently used in metabolic engineering as the wild-type strain. However, its two mutations, ilvG and rph-1 provide a negative effect on culture growth. The "polar effect" of rph-1 decreases the level of pyrE expre
Autor:
Larisa G. Airich, Sergey V. Mashko, Evgeniya M. Kiseleva, N. I. Minaeva, Alexander A. Krylov, Irina Vladimirovna Biryukova
Publikováno v:
Microbial Physiology. 18:1-13
PykF is one of two pyruvate kinases in Escherichia coli K-12. λPL was convergently integrated into the chromosome of the MG1655 strain, downstream of pykF, face-to-face with its native promoter. In the presence of λcIts857, efficient pykF ts-silenc
Autor:
Zinaida Polonskaya, A. Yu. Gulevich, S. V. Mashko, Irina Vladimirovna Biryukova, Danila Vadimovich Zimenkov, Alexander A. Krylov, V. Yu. Ermishev, N. I. Minaeva, A. Yu. Skorokhodova
Publikováno v:
Molecular Biology. 43:505-514
A new method for the construction of translationally coupled operons in a bacterial chromosome was developed on the basis of the recombineering approach. The method includes the in vitro construction of an artificial operon with an efficiently transl
Autor:
N. I. Minaeva, Vera Georgievna Doroshenko, A. Yu. Gulevich, Irina Vladimirovna Biryukova, S. V. Mashko, A. Yu. Skorokhodova, Danila Vadimovich Zimenkov, Joanna I. Katashkina
Publikováno v:
Molecular Biology. 39:719-726
A new method of constructing a set of bacterial cell clones varying in the strength of a promoter upstream of the gene of interest was developed with the use of Escherichia coli MG1655 and lacZ as a reporter. The gist of it lies in constructing a set
Publikováno v:
Genetika. 46(3)
MG1655 of Escherichia coli K-12 is frequently used in metabolic engineering as the wild-type strain. However, its two mutations, ilvG and rph-1 provide a negative effect on culture growth. The "polar effect" of rph-1 decreases the level of pyrE expre
Autor:
A Iu, Gulevich, A Iu, Skorokhodova, V Iu, Ermishev, A A, Krylov, N I, Minaeva, Z M, Polonskaia, D V, Zimenkov, I V, Biriukova, S V, Mashko
Publikováno v:
Molekuliarnaia biologiia. 43(3)
The new method of translational-coupled operons construction in bacterial chromosome has been developed on the basis of recombineering approach. It includes construction in vitro of the artificial operon with efficiently translated proximal cistron f
Autor:
Zh I, Katashkina, A Iu, Skorokhodova, D V, Zimenkov, A Iu, Gulevich, N I, Minaeva, V G, Doroshenko, I V, Biriukova, S V, Mashko
Publikováno v:
Molekuliarnaia biologiia. 39(5)
The new method of construction of the set of E. coli clones, differing in the promoter strength upstream the gene of interest, has been developed and tested using native E. coli MG 1655 lacZ gene as the reporter. This method includes the construction
Autor:
Aleksandra Yu Skorokhodova, Sergey V. Mashko, Irina Vladimirovna Biryukova, Danila Vadimovich Zimenkov, Evgeny R Gak, N. I. Minaeva
Publikováno v:
BMC Biotechnology, Vol 8, Iss 1, p 63 (2008)
BMC Biotechnology
BMC Biotechnology
Background The development of modern producer strains with metabolically engineered pathways poses special problems that often require manipulating many genes and expressing them individually at different levels or under separate regulatory controls.