Zobrazeno 1 - 10
of 34
pro vyhledávání: '"N. G. Wrigley"'
Autor:
N. G. Wrigley, W. Baschong
Publikováno v:
Journal of electron microscopy technique. 14(4)
Fab-colloidal gold labelling in conjunction with negative staining and high-resolution electron microscopy was used for targeting single protein units in regular arrays. These were bacteriophage T4 polyheads with Fab-Au2.5, and a specific antibody bi
Publikováno v:
FEBS Letters. 80:1-4
1. Introduction We have reported the occurrence in human erythro- cytes of a specific NADP (H) -binding protein, desig- nated FX [l] , which was recently purified by affinity chromatography and obtained in a homogeneous form [2]. Despite its unusuall
Publikováno v:
Journal of Biological Chemistry. 255:9458-9464
Antibodies prepared against biotin linked to serum albumin have been purified by affinity chromatography using lipoyl-Sepharose (Harmon, F. (1980) Anal. Biochem. 103, 58-63). These antibodies have been used to study the structure of the biotin-contai
Autor:
N G Wrigley
Publikováno v:
Journal of Biological Chemistry. 252:1500-1504
In this paper we show that the native form of transcarboxylase may be a species which has six rather than three subunits attached to the central subunit. We have designated this form as the 26 S enzyme. Electron micrographs support the view that the
Publikováno v:
Journal of Biological Chemistry. 254:1740-1747
Electron microscopic studies of chicken liver pyruvate carboxylase conducted under a variety of conditions show that this enzyme has an overall rhombic appearance and is comprised of four nonspherical subunits. The square planar tetramers originally
Publikováno v:
Biochemistry. 18:2197-2203
Autor:
N. G. Wrigley
Publikováno v:
Journal of General Virology. 6:169-173
I recently reported in this journal some observations on the structure of Sericesthis iridescent virus (SIV) (Wrigley, 1969). In this short communication I report similar observations on the closely related Tipula iridescent virus (TIV) and interpret
Autor:
N. G. Wrigley
Publikováno v:
Journal of General Virology. 5:123-134
Summary Purified suspensions of Sericesthis iridescent virus were treated with a nasal decongestant, negatively stained and examined in the electron microscope. The outer icosahedral surface of the virus particles showed morphological subunits appare
Publikováno v:
Virology. 51:525-529
Autor:
N. G. Wrigley, N. Michael Green, Birgit Jacobson, Harland G. Wood, R. C. Valentine, Fazal Ahmad
Publikováno v:
Journal of Biological Chemistry. 247:6284-6298
The molecular weights of transcarboxylase and its constituent subunits have been determined by sedimentation equilibrium and by gel electrophoresis and the organization of the subunits has been deduced from electron micrographs. The intact enzyme (mo