Zobrazeno 1 - 10
of 248
pro vyhledávání: '"N. Emaus"'
Publikováno v:
Plant Methods, Vol 18, Iss 1, Pp 1-14 (2022)
Abstract Background Plant DNA isolation and purification is a time-consuming and laborious process relative to epithelial and viral DNA sample preparation due to the cell wall. The lysis of plant cells to free intracellular DNA normally requires high
Externí odkaz:
https://doaj.org/article/d3cf644e9f5746d0a10a03532dfa4a30
Autor:
Arianna Marengo, Cecilia Cagliero, Barbara Sgorbini, Jared L. Anderson, Miranda N. Emaus, Carlo Bicchi, Cinzia M. Bertea, Patrizia Rubiolo
Publikováno v:
Plant Methods, Vol 15, Iss 1, Pp 1-11 (2019)
Abstract Background Nowadays, there is an increasing demand for fast and reliable plant biomolecular analyses. Conventional methods for the isolation of nucleic acids are time-consuming and require multiple and often non-automatable steps to remove c
Externí odkaz:
https://doaj.org/article/d2bdd72df18247d3981125a8c8843c91
Akademický článek
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Autor:
Miranda N. Emaus, Jared L. Anderson
Publikováno v:
Analytical and Bioanalytical Chemistry. 414:277-286
Sequence-specific DNA extractions have the potential to improve the detection of low-abundance mutations from complex matrices, making them ideal for circulating tumor DNA analysis during the early stages of cancer. Ion-tagged oligonucleotides (ITOs)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f389e7406a819752b8c0f2ed0f9aeae5
https://doi.org/10.1007/s00216-021-03216-8
https://doi.org/10.1007/s00216-021-03216-8
Akademický článek
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Fluorescence quenching of the SYBR Green I-dsDNA complex by in situ generated magnetic ionic liquids
Autor:
Jared L. Anderson, María J. Trujillo-Rodríguez, Kalyan Santra, Anthony Song, Ashley N. Bowers, Miranda N. Emaus, Jacob W. Petrich
Publikováno v:
Analytical and Bioanalytical Chemistry. 412:2743-2754
Magnetic ionic liquids (MILs) with metal-containing cations are promising extraction solvents that provide fast and high efficiency extraction of DNA. Hydrophobic MILs can be generated in situ in a methodology called in situ dispersive liquid-liquid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c808c2b008672b1eaff2f8cd3974d953
https://doi.org/10.1007/s00216-020-02538-3
https://doi.org/10.1007/s00216-020-02538-3
Publikováno v:
Analytica Chimica Acta. 1094:1-10
Detection of circulating tumor DNA (ctDNA) presents several challenges due to single-nucleotide polymorphisms and large amounts of background DNA. Previously, we reported a sequence-specific DNA extraction procedure utilizing functionalized oligonucl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::844a2f82646b505b7299308a666d1542
https://doi.org/10.1016/j.aca.2019.10.057
https://doi.org/10.1016/j.aca.2019.10.057
Publikováno v:
Analytica Chimica Acta. 1068:1-10
Circulating tumor DNA (ctDNA) is a source of mutant DNA found in plasma and holds great promise in guiding cancer diagnostics, prognostics, and treatment. However, ctDNA fragments are challenging to detect in plasma due to their low abundance compare
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0379bc56b28e51a4c89c7c15bc1f51c
https://doi.org/10.1016/j.aca.2019.04.005
https://doi.org/10.1016/j.aca.2019.04.005
Autor:
Miranda N. Emaus, Jared L. Anderson
Publikováno v:
Analytical and bioanalytical chemistry. 412(29)
Conventional DNA sample preparation methods involve tedious sample handling steps that require numerous inhibitors of the polymerase chain reaction (PCR) and instrumentation to implement. These disadvantages limit the applicability of conventional ce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a85250830c66d5238206a37fd271e3e7
https://pubmed.ncbi.nlm.nih.gov/32918171
https://pubmed.ncbi.nlm.nih.gov/32918171