Zobrazeno 1 - 10
of 244
pro vyhledávání: '"N. Catsimpoolas"'
Publikováno v:
International Journal of Protein Research. 3:63-71
Electrometric and spectrophotometric titration curves of glycinin were obtained at 0.4 ionic strength and in the presence of 6M guanidine hydrochloride and 6M urea. The electrometric hydrogen ion titration curves were irreversible in the pH range 6.5
Publikováno v:
International Journal of Protein Research. 2:277-284
Optical rotatory dispersion (ORD) studies and infrared spectra in deuterium oxide indicate that native glycinin exhibits mainly a β-conformation structure. Ultraviolet difference spectra showed that urea ami guanidine hydrochloride at increasing con
Autor:
N. Catsimpoolas, J. Marti
Publikováno v:
Proceedings. 25th Annual Simulation Symposium.
Publikováno v:
Antonie van Leeuwenhoek. 41:69-79
Scanning isoelectric focusing has been employed for continuous monitoring of the isoelectric spectrum of highly purified cholera enterotoxin in 4% polyacrylamide gels containing 2% ampholytes pH 3-10. The resolution obtained by this technique is of h
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 351:196-204
Kinetic methods are described for the measurement of the minimal focusing time (tMF) of amphoteric molecules subjected to isoelectric focusing. The parameter tMF is defined as the time required for a particular compound to migrate to its isoelectric
Publikováno v:
International Archives of Allergy and Immunology. 63:258-265
Preparative density gradient electrophoresis has been employed for the separation of BALB/c mouse spleen T and B lymphocytes, on the basis of their surface charge. The high mobility cells were found to be predominantly T lymphocytes, whereas the low
Publikováno v:
The Journal of Immunology. 117:1949-1952
A new preparative method is described for the separation and direct electrophoretic comparison of the distribution profile of radioisotopically labeled cells. The cell populations to be compared are labeled separately with different radioisotopes (e.
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 175:76-81
Soybean trypsin inhibitor was isolated from commercial crude soybean trypsin inhibitor and soybean whey protein by isoelectric focusing in the region between pH 3 and pH 10. The inhibitor was isoelectrically focused at pH 4.5. Disc electrophoresis in
Autor:
N. Catsimpoolas, E. Leuthner
Publikováno v:
Analytical Biochemistry. 31:437-447
Immunization of rabbits with Kunitz soybean trypsin inhibitor results in the formation of antisera that can be utilized for the detection and quantitative determination of this protein. The trypsin inhibitor was isolated from a commercial preparation
Autor:
John L. Wood, N. Catsimpoolas
Publikováno v:
Journal of Biological Chemistry. 239:4132-4137