Zobrazeno 1 - 10
of 13
pro vyhledávání: '"N V, Anikeĭcheva"'
Autor:
O T, Samko, A A, Kalugin, M A, El'darov, I V, Karpychev, N V, Anikeĭcheva, E B, Khoroshutina, K G, Skriabin, G I, Librik, N N, Sokolov
Publikováno v:
Bioorganicheskaia khimiia. 20(12)
New site-specific endonucleases BciBI and BciBII have been detected in Bacillus circulans. The enzymes were purified by fractionation of cell-free extract with polyethylene imine and ammonium sulphate (40-80% of saturation) followed by chromatography
Autor:
N N, Sokolov, M A, El'darov, N V, Anikeĭcheva, I V, Karpychev, O T, Samko, A B, Fitsner, A A, Kalugin, E B, Khoroshutina, K G, Skriabin
Publikováno v:
Bioorganicheskaia khimiia. 18(1)
A new restriction endonuclease BbvBI free from contaminating nonspecific nucleases and phosphatases was isolated from the Bacillus brevis cells. The enzyme was purified by fractionating the sonicated cell-free extract in a two-phase PEG/dextran syste
Autor:
N N, Sokolov, M A, El'darov, N V, Anikeĭcheva, I V, Karpychev, O T, Samko, A B, Fitsner, A A, Kalugin, E B, Khoroshutina, K G, Skriabin
Publikováno v:
Bioorganicheskaia khimiia. 17(9)
A new site-specific endonuclease was detected in toluene lysates of Bacillus coagulans AUCM B-732 and designated as BcoAI. The enzyme was purified by fractionation of the cell-free extract in the two-phase PEG/dextran system followed by chromatograph
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 85(4)
Escherichia coli SK cells are not colicinogenic, but possess multiple resistance to antibiotics (tetracycline, kanamycin, penicillin, polymyxin, ampicillin). Numerous variations, sensitive to one or several of the antibiotics listed were obtained by
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (5)
Specific endonuclease activities have been found it two Pseudomonas aeruginosa strains. Isolation and purification of enzymes and determining their specific activities have permitted one to find out that PaeI is an isoshizomer of SphI and digests the
Publikováno v:
Voprosy meditsinskoi khimii. 35(6)
Electrophoretic analysis of products obtained after hydrolysis of phage lambda DNA by means of restrictase Pae I was carried out after preincubation of DNA or the enzyme with Mg2+ as well as after preincubation of DNA simultaneously with the enzyme a
Publikováno v:
Voprosy meditsinskoi khimii. 25(4)
Superspiralized coiled plasmide DNA were found in cells E. coli CK. One of plasmides pEco CK-1 had a size 21.2 micron that corresponded to molecular mass 43.7 megadaltons, another plasmide pEco CK-2 with the size 14.2 micron had a molecular weight 29
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 102(12)
5,5'-dithiobis-2-nitrobenzoic acid, N-ethylmaleimide, and parachloromercuribenzoate have been demonstrated to inhibit the activity of restrictases PaeI and PaeII from Ps. aeruginosa bacterial cells. Restrictase PaeII was more sensitive to the action
[Relations between the condition of growth and activity of DNA-methylases of Mycobacteria butyricum]
Publikováno v:
Voprosy meditsinskoi khimii. 28(5)
Conditions for cultivation of Mycobacteria butiricum were studied. Optimal conditions were selected to maintain maximal rate of DNA-metylase activity keeping of unspecific nuclease activity in cells at a low level. Analyses of plots of the time- depe
Publikováno v:
Zhurnal mikrobiologii, epidemiologii i immunobiologii. (9)
The search for restrictases in 154 strains belonging to 104 species of 32 genera of microorganisms has been carried out by the method of rapid toluene assay. In 10 strains the activity of endonucleases specifically fragmenting the DNA of phage lambda