Leptin attenuates the acute effects of centrally administered neuropeptide Y on somatotropin but not gonadotropin secretion in ovariectomized cows

Autor: Marcel Amstalden, Duane H. Keisler, M.R Garcia, Arieh Gertler, Gary L. Williams, N Raver

Publikováno v: Domestic Animal Endocrinology. 26:189-200

We tested the hypothesis that recombinant ovine leptin would attenuate the acute effects of neuropeptide Y (NPY) on secretion of GH and gonadotropins (LH and FSH) in cows. Ovariectomized cows (n=6) fitted with third ventricle guide cannulas were assi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0c038b6cea7887e97840acc326bc857
https://doi.org/10.1016/j.domaniend.2003.10.003

A chicken leptin-specific radioimmunoassay

Autor: N Raver, Veerle Bruggeman, M Onagbesan, Jean Djiane, Mohammed Taouis, Eddy Decuypere, Sami Dridi, J Williams, Arieh Gertler

Publikováno v: Domestic Animal Endocrinology
Domestic Animal Endocrinology, Elsevier, 2000, 18, pp.325-335
Domestic Animal Endocrinology, Elsevier, 2000, 18 (3), pp.325-335. ⟨10.1016/s0739-7240(00)00053-9⟩

International audience; Recombinant chicken leptin was used to produce an antiserum in order to develop a specific and sensitive radioimmunoassay (RIA) for chicken leptin in plasma and serum. We have used either murine or chicken leptin as tracer and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55bb0b95ffd71b2ca021e28038b8f473
https://doi.org/10.1016/s0739-7240(00)00053-9

Mapping leptin-interacting sites in recombinant leptin-binding domain (LBD) subcloned from chicken leptin receptor

Autor: Miriam Friedman-Einat, Oded Livnah, Eugene E. Gussakovsky, Arieh Gertler, N Raver, Leonora Niv-Spector, J. Grosclaude

Publikováno v: Biochemical Journal
Biochemical Journal, Portland Press, 2005, 390 (2), pp.475-484. ⟨10.1042/BJ20050233⟩

The binding domain of the chicken leptin receptor [chLBD (chicken leptin-binding domain)], subcloned from the full-size chicken leptin receptor and prepared in an Escherichia coli system, was subjected to site-directed mutagenesis to identify the ami

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34421943885fe48f4bc904f44b5ab558
https://hal.inrae.fr/hal-02682473

Attenuation by leptin of the effects of fasting on ovarian function in hens (Gallus domesticus)

Autor: T Jacek, A Gertler, J. Rzasa, J Proudman, N Raver, A Hrabia, M Mika, A. Sechman, S Cassy, H Paczoska-Eliasiewicz, M Proszkowiec

Publikováno v: Reproduction-Cambridge-Supplement
Reproduction-Cambridge-Supplement-, Society for Reproduction and Fertility, 2003, 126, pp.739-751

Thirty-four-week-old laying hens received injections of recombinant chicken leptin to assess the role of leptin in avian ovarian function. In the first experiment, the hens (n=60) were divided into three groups: (i). fed ad libitum; (ii). fasted; and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::134a4bc4668d8f922354f2f0a53321f2
https://hal.inrae.fr/hal-02678947

Subcloning, expression, purification, and characterization of recombinant human leptin-binding domain

Autor: Radha G. Krishna, N Raver, Eugene E. Gussakovsky, Suzan Shochat, Orly Dym, Oded Livnah, Yael Sandowski, Menachem Rubinstein, Arieh Gertler

Publikováno v: The Journal of biological chemistry. 277(48)

A subdomain of the human leptin receptor encoding part of the extracellular domain (amino acids 428 to 635) was subcloned, expressed in a prokaryotic host, and purified to homogeneity, as evidenced by SDS-PAGE, with over 95% monomeric protein. The pu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84b06d15e32393f48bbc4d2bef997177
https://pubmed.ncbi.nlm.nih.gov/12226096