Zobrazeno 1 - 10
of 87
pro vyhledávání: '"N N, Ugarova"'
Publikováno v:
Антибиотики и Химиотерапия, Vol 54, Iss 7-8, Pp 8-12 (2020)
Rapid testing of pathogen susceptibility to antibiotics is of great practical value for rational chemotherapy of pyoinflammatory deseases and postoperative complications of microbial etiology. The standard microbiological methods i.e. the disk diffus
Externí odkaz:
https://doaj.org/article/290ae6db53644148a32cd7e952a0d843
Autor:
G. Yu. Lomakina, N. N. Ugarova
Publikováno v:
Moscow University Chemistry Bulletin. 77:42-47
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::32db9615d7a039f7b25e5565626e4cec
https://doi.org/10.3103/s0027131422010059
https://doi.org/10.3103/s0027131422010059
Publikováno v:
Moscow University Chemistry Bulletin. 65:202-205
Lytic bacteriophages and “Disruptor” 4610 filters made of nanoalumina fibers were used for specific determination of determination of bacterial contamination by ATP assay. As a model system the system of bacteriophage T4-E. coli B was used as a m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8e91ca9cf616973d2960b4ab64aef983
https://doi.org/10.3103/s0027131410030181
https://doi.org/10.3103/s0027131410030181
Autor:
N. N. Ugarova
Publikováno v:
Moscow University Chemistry Bulletin. 65:139-143
The results of Luciola mingrelica firefly luciferase stabilization by genetic engineering methods are reviewed. The Cys62, Cys146, and Cys164 to Ser mutant enzymes with an enhanced thermostability and lower sensitivity to dithiothreitol were obtained
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::54cb26973628336336e0d5e2c7dc4efd
https://doi.org/10.3103/s0027131410030065
https://doi.org/10.3103/s0027131410030065
Autor:
M. I. Koksharov, N. N. Ugarova
Publikováno v:
Moscow University Chemistry Bulletin. 64:18-22
Luciferase of lighting bugs finds wide application in a number of fields in biotechnology and molecular biology, but use of luciferase is often limited by its fast inactivation at elevated temperatures. As a result of four sequential cycles of random
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::17c2ee7e893099b774c68901f61cf6c3
https://doi.org/10.3103/s0027131409010040
https://doi.org/10.3103/s0027131409010040
Publikováno v:
Moscow University Chemistry Bulletin. 63:67-70
ATP reagents containing 2–20% of ethylene glycol, glycerol, or PEG-8000 have been prepared. Polyols inhibit the activity of the ATP reagents by 20–40%, and addition of 0.75 mM Triton X-100 prevents this inhibition. The optimal polyol concentratio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e8bafffad9be5472aefe1d8655560977
https://doi.org/10.3103/s0027131408020041
https://doi.org/10.3103/s0027131408020041
Autor:
N. N. Ugarova, L. Yu. Brovko
Publikováno v:
Russian Chemical Bulletin. 50:1752-1761
Bioluminescence spectra for various native and mutant luciferases from fireflies and beetles were analyzed in the light of the known theoretical concepts on the influence of the microenvironment of the emitter on its emission spectra. The mechanism f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::afed840513819f34cee85cde95697b4f
https://doi.org/10.1023/a:1014365609421
https://doi.org/10.1023/a:1014365609421
Autor:
N. N. Ugarova, L.Yu. Brovko, Nikolai I. Koroteev, E. Yu. Cherednikova, Ekaterina Dementieva, A. Yu. Chikishev
Publikováno v:
Scopus-Elsevier
An experiment was proposed and accomplished that was based on the hypothesis of the dissociation of the luciferase-luciferin complex in photoexcitation. A pump-probe experiment was performed with the use of picosecond laser pulses and was based on th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2496eb1e2abbcde80ca155a06966824
https://doi.org/10.1002/(sici)1520-6343(1999)5:6<378::aid-bspy7>3.0.co
https://doi.org/10.1002/(sici)1520-6343(1999)5:6<378::aid-bspy7>3.0.co
Publikováno v:
Analytical Letters. 27:2949-2959
Bioluminescent method for β-galactosidase assay is proposed with sensitivity as high as 0.25 fmol/1 with the help of bioluminogenic substrate – firefly luciferin derivative. Method was applied to immunoassay of foot-and-mouth disease viral antigen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::48fdef6f29c6dd62dc96f8b32f25393f
https://doi.org/10.1080/00032719408000303
https://doi.org/10.1080/00032719408000303
Publikováno v:
Journal of Photochemistry and Photobiology B: Biology. 22:203-209
Fluorescence excitation and emission spectra and fluorescence decay curves of firefly luciferase complexes with oxyluciferin (LO), luciferin (LH2), 6′-methoxyluciferin (MeOLH2) and 2-cyano-6-hydroxy-benzothiazole (BT) were obtained in aqueous (pH 2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ab35b15c9b4f1e4a7aa38c163285bd93
https://doi.org/10.1016/1011-1344(93)06970-e
https://doi.org/10.1016/1011-1344(93)06970-e