Zobrazeno 1 - 10
of 14
pro vyhledávání: '"N N, Lemeshko"'
Autor:
A S, Vladyko, S I, Bystrova, I V, Malakhova, L Ia, Kunitskaia, N N, Lemeshko, A G, Kras'ko, A T, Kozhich, I S, Lukashevich
Publikováno v:
Voprosy virusologii. 38(1)
Nucleocapsid protein of Lassa virus contains 4 epitope sites. Three of them were localized in amino acid position 123-1 27, 337-346, and 518-527. Monoclonal antibody 1108 specific to Lassa virus NP-protein reacted with 123-127 synthetic peptide in so
Publikováno v:
Voprosy virusologii. 37(1)
The immunogenic properties of Lassa virus GP1, GP2, and NP polypeptides were studied in rabbits. Lassa virus NP polypeptide, in contrast to GP1 and GP2 polypeptides, was shown to induce the highest titres of antibodies determined by IFA and ELISA tes
Publikováno v:
Voprosy virusologii. 36(5)
Comparative studies of two variants of the enzyme-linked immunosorbent assay (ELISA) were carried out to determine the sensitivity of the detection of Marburg virus antigens in Vero cells. Both competitive and two-antibody ELISA variants detected as
Autor:
P G, Rytik, G, van der Groen, V F, Eremin, N D, Kolomiets, S A, Popov, P, Nijs, V, Willems, G, Verkauteren, Iu G, Il'kevich, N N, Lemeshko
Publikováno v:
Voprosy virusologii. 35(5)
Biological properties of an AIDS agent first isolated from a native citizen in the USSR are presented. The source of the virus was a young Byelorussian woman who in the near past had had sexual contacts with a citizen from one of the Central Africa c
Publikováno v:
Voprosy virusologii. 35(5)
Recombinant monoclonal antibodies to Lassa virus were produced. The reactivity of the monoclonal antibodies was studied by indirect fluorescence antibody (IFA) technique, enzyme-linked immunosorbent assay (ELISA), and radioimmunoprecipitation method.
Autor:
I S, Lukashevich, E A, Tkachenko, N N, Lemeshko, G V, Rezapkin, T A, Stel'makh, A Ia, Pashkov, A S, Vladyko, T K, Dzagurova, T V, Shkolina, E P, Scheslenok
Publikováno v:
Voprosy virusologii. 35(1)
Virus-specific proteins G1, G2, and N with molecular weights of 70, 55-57, and 50 kilodaltons, respectively, were detected by radioimmunodiffusion tests in VERO E-6 cells infected with strains of virus of hemorrhagic fever with renal syndrome (HFRS)
Publikováno v:
Voprosy virusologii. 29(5)
The effect of inhibitors of mRNA synthesis (actinomycin D and alpha-amanitin) and DNA replication (mitomycin C and ethidium bromide) on Machupo virus reproduction was studied. Actinomycin D (1.0-4.0 micrograms/ml) and alpha-amanitin (10.0 micrograms/
Publikováno v:
Acta virologica. 33(2)
14C-labelled Lassa virus was purified by isopycnic centrifugation and split to subvirion fractions. The purified virus was treated with nonionic detergents Nonidet P-40 (NP-40) and with octylglycoside. After ultracentrifugation in urographin density
Publikováno v:
Voprosy virusologii. 30(4)
Machupo virus labeled with radioactive precursors of RNA or protein synthesis was purified by isodensity ultracentrifugation in sucrose concentration gradient. The purified virus was disrupted with NP-40 nonionic detergent in the presence of 1 M KCl,
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (5)
Some properties of monoclonal antibodies to the Lassa virus have been characterized. The competitive immunoenzyme analysis has revealed the presence of at least three antigens in the Lassa virus nucleoprotein.