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pro vyhledávání: '"N M, Arzamazova"'
Autor:
N M, Arzamazova, E A, Arystarkhova, N M, Gevondyan, N M, Luneva, R G, Efremov, N A, Aldanova, V A, Nesmeyanov, N N, Modyanov
Publikováno v:
Progress in clinical and biological research.
Publikováno v:
Bioorganicheskaia khimiia. 13(5)
A procedure for highly selective isolation of tryptophan- and cysteine-containing peptides from protein hydrolysates has been developed on the basis of covalent chromatography. It includes incorporation of a thiol group into the tryptophan residues b
Publikováno v:
Bioorganicheskaia khimiia. 13(1)
The Na+, K+-ATPase's beta-subunit immobilized on thiol-glass was hydrolyzed with trypsin. Over 25 peptides covering ca. 90% of the protein polypeptide chain were isolated from the digest by HPLC and characterized. Structural analysis allowed us to lo
Publikováno v:
Bioorganicheskaia khimiia. 11(12)
The selective tryptic digestion of the native membrane-bound enzyme was carried out under conditions that provide the extensive hydrolysis of hydrophilic regions of the alpha-subunit into small fragments and allow to preserve the integrity of the bet