Zobrazeno 1 - 10
of 12
pro vyhledávání: '"N K, Daniliuk"'
Autor:
A A, Il'ichev, L I, Karpenko, N A, Nekrasova, L R, Lebedev, G M, Ignat'ev, A P, Agafonov, P A, Belavin, S S, Seregin, N K, Daniliuk, S I, Bazhan
Publikováno v:
Vestnik Rossiiskoi akademii meditsinskikh nauk. (1)
We used, within the case study, virus-like particles (VLP) and attenuated strains of salmonella for the delivery of HIV-1 DNA vaccine encoding the multiepitope CTL-immunogene. The immunogenicity of the thus obtained vaccine constructions was comparat
Publikováno v:
Bioorganicheskaia khimiia. 21(5)
Chemical-enzymatic synthesis and cloning of a gene for a human anaphylatoxin C5a analog were carried out. Recombinant plasmid pRC5a providing the expression of the synthetic gene in the Escherichia coli cells was obtained. The biological activity of
Publikováno v:
Molekuliarnaia biologiia. 27(1)
Recombinant plasmids were constructed for the efficient expression in E. coli cells of the human interleukin-2 (HIL-2) gene and two its mutant analogues obtained by of chemical-enzymic synthesis and polymerase chain reaction (deletion of 14 C-termina
Autor:
N K, Daniliuk, S V, Seregin, A N, Siniakov, O I, Serpinskiĭ, I N, Babkina, M A, Urmanova, V A, Riabinin, S G, Pozdniakov
Publikováno v:
Bioorganicheskaia khimiia. 17(6)
Artificial DNA fragments encoding human interleukin-2 (133 a.a.) and its analogue (deletion of 14 C-terminal a.a.) were prepared by means of the DNA polymerase I mediated extension of synthetic polynucleotides having short overlapping sequences at th
Publikováno v:
Bioorganicheskaia khimiia. 17(1)
By cloning synthetic oligonucleotides into pUC18 plasmid, pFH123--pFH127 plasmids have been constructed. Their polylinker area, along with sites of widely used restriction endonucleases, contains two pairs each of FokI and HgaI sites in the opposite
Publikováno v:
Bioorganicheskaia khimiia. 16(6)
Chemical-enzymatic synthesis and cloning of the DNA fragment coding for the human interleukin-2 signal sequence was accomplished. A hybrid plasmid pSIL-2 containing the gene of the human interleukin-2 with this signal sequence was constructed for eff
Publikováno v:
Doklady Akademii nauk SSSR. 310(4)
Publikováno v:
Doklady Akademii nauk SSSR. 313(2)
Publikováno v:
Bioorganicheskaia khimiia. 12(5)
A series of oligonucleotides, including two polynucleotides of 33 bases long, were synthesized by a solid-phase phosphotriester method. Potassium salt of 3-nitro-1,2,4-triazole in the presence of 18-crown-6 ether was used as nucleophilic catalyst. Th
Publikováno v:
Bioorganicheskaia khimiia. 12(9)
A modification to the Maxam-Gilbert method is proposed that involves precipitation of the nucleotide material with the acetone solution of lithium perchlorate after the completion of chemical reactions to remove the reagents. Modification of cytosine