Zobrazeno 1 - 10 of 22 pro vyhledávání: '"N Ia, Orlov"'
1
[Activation of bovine retinal rod outer segment cGMP-specific phosphodiesterase by the transducin-GTP complex in a physiologically significant range of free calcium ion concentrations]
Autor: O V, Petrukhin, T G, Orlova, A R, Nizvetskiĭ, N Ia, Orlov
Publikováno v: Biofizika. 59(5)
The kinetic behavior of cGMP-specific phosphodiesterase in a totally bleached bovine retinal rod outer segment suspension was studied by the pH-metric method at high and low concentrations of free calcium ions (≈ 100 μM and 10 nM, respectively). T
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::30b567a29c045cc3b3547e4833770f27
https://pubmed.ncbi.nlm.nih.gov/25730965
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2
[The phosphorylation state of transducin beta-subunits]
Autor: D N, Orlov, A R, Nezvetskiĭ, T G, Orlova, O V, Petrukhin, N Ia, Orlov
Publikováno v: Biofizika. 59(5)
The supposition that nucleoside diphosphate kinase is the enzyme that phosphorylates transducin beta-subunits on one of the histidine residues (His-266) has been analyzed. It stands the reason that 1) this enzyme is multifunctional and plays in parti
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::052af524a928ba4a3eac57823d9f9710
https://pubmed.ncbi.nlm.nih.gov/25730962
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3
[Purification of transducin betagamma-subunit complex from isotonic extracts of bovine retinal rod outer segments]
Autor: D N, Orlov, T G, Orlova, A R, Nezvetskiĭ, N Ia, Orlov
Publikováno v: Biofizika. 55(6)
A method for obtaining a free complex of transducin betagamma-subunits from bovine retinal rod outer segments in a highly purified state has been suggested.
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e681fe674129179031129e46a6064813
https://pubmed.ncbi.nlm.nih.gov/21268340
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4
[Nucleoside diphosphate kinase and GTP-binding proteins. Possible mechanisms of coupling]
Autor: D N, Orlov, N Ia, Orlov
Publikováno v: Biofizika. 53(6)
The results of numerous investigations during the last 20 years have shown that nucleoside diphosphate kinase (NDP kinase) is a multifunctional protein. In this paper, the current data are analyzed indicating that one of the possible mechanisms by wh
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::df9a08df482c4c86278cdda6136233a4
https://pubmed.ncbi.nlm.nih.gov/19137672
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5
[Thermostable extracellular cyclic nucleotide phosphodiesterase from Physarum polycephalum plasmodium]
Autor: A R, Nezvetskiĭ, T G, Orlova, S I, Beĭlina, N Ia, Orlov
Publikováno v: Biofizika. 51(5)
The cyclic nucleotide phosphodiesterase secreted by Physarum polycephalum plasmodium into extracellular medium has been partially purified by DEAE cellulose chromatography, ultrafiltration, and HPLC. The results obtained by gel filtration, HPLC, elec
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::0a6b16530d60fe8ec396e076c14e7c5b
https://pubmed.ncbi.nlm.nih.gov/17131817
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6
[Calcium-dependent interaction of transducin with calmodulin-sepharose]
Autor: V M, Grishchenko, T G, Orlova, A A, Freĭdin, N Ia, Orlov
Publikováno v: Biofizika. 51(5)
It has been shown by affinity chromatography on calmodulin-sepharose that transducin, a G protein of bovine retinal rod outer segments interacts with Ca(2+)-calmodulin. This result assumes that the main part of calmodulin in dark retinal rod outer se
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ec4a45a1cefcaa4afc1c8d77c8a48c59
https://pubmed.ncbi.nlm.nih.gov/17131818
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7
[Fast photo-induced changes in the light scattering of vertebrate photoreceptor membrane preparations. Study of suspensions of vesicles obtained by ultrasonic treatment of the outer segments of retinal rods]
Autor: N Ia, Orlov, A G, Gasparian, E E, Fesenko
Publikováno v: Biofizika. 25(1)
By light scattering method it was shown, that vesicles, obtained by sonication of rod outer segment membranes in different ionic media rapidly and irreversibly increased their volume under bleaching of rhodopsin. Apparently, this volume change was ca
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5f4a20b5f5764f4c5af627385c287c78
https://pubmed.ncbi.nlm.nih.gov/6154486
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8
[Molecular mechanisms of photoreception. IV. Photoregeneration of rhodopsin from metarhodopsin II using the artificial lipid membrane method for detection of intermediate steps of this reaction]
Autor: N Ia, Orlov, E E, Fesenko
Publikováno v: Molekuliarnaia biologiia. 15(6)
It was shown that photoregeneration of rhodopsin from metarhodopsin II takes place in bovine retinal rod outer segment disc membrane fragments. Photoregenerated rhodopsin is identical to unbleached rhodopsin in spectra and in stability to NH2OH. More
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::62f694d44c9316278290f712f3322e5b
https://pubmed.ncbi.nlm.nih.gov/7322116
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10
[Protein kinase activity of bovine retina rod outer segments]
Autor: E E, Fesenko, N Ia, Orlov
Publikováno v: Molekuliarnaia biologiia. 14(4)
Dark-adapted pure bovine rod outer segments (ROS) (A280/A500--2.1) can be phosphorylated in the presence of [gamma-32P]ATP and [gamma-32P]GTP. The constant levels of phosphorylation, reached within 10--15 min, are 100 +/- 30 pmol 32P/nmol of rhodopsi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5b409b41180e5b0f37e5a45f1125e94f
https://pubmed.ncbi.nlm.nih.gov/6252443
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