Výsledky vyhledávání

An experimental model of intracranial hemorrhage during thrombolytic therapy with t-PA

Autor: N F, Paoni, H G, Steinmetz, N, Gillett, C J, Refino, J M, Badillo, S, Bunting

Publikováno v: Thrombosis and haemostasis. 75(5)

Multiple clinical trials have proven that thrombolytic therapy is an effective treatment for acute myocardial infarction. Spontaneous intracranial hemorrhage (ICH) occurs in a small percentage of patients as a result of the treatment. The etiology of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::29ce8c8f1f42d433fa9785dc46ca856f
https://pubmed.ncbi.nlm.nih.gov/8725730

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Substitution of serine or threonine at position 473 of tissue-type plasminogen activator increases its stability in plasma

Autor: M, Alibhai, A M, Chow, N F, Paoni

Publikováno v: Thrombosis and haemostasis. 75(1)

Inactivation by slow acting inhibitors in plasma is of little consequence for thrombolysis with wild type t-PA, since it is rapidly cleared from the blood stream and constantly replenished through infusion. However, it becomes increasingly important

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::dd84ce26aa87787c61e05767467da373
https://pubmed.ncbi.nlm.nih.gov/8713788

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A faster-acting and more potent form of tissue plasminogen activator

Autor: J Lai, Luis C. Pena, L. Berleau, Bruce Keyt, C Pater, J Ogez, C J Refino, Alice M. Chow, N F Paoni, Hung V. Nguyen

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America. 91(9)

Current treatment with tissue plasminogen activator (tPA) requires an intravenous infusion (1.5-3 h) because the clearance of tPA from the circulation is rapid (t 1/2 approximately 6 min). We have developed a tPA variant, T103N,N117Q, KHRR(296-299)AA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1b49fb3a4f0dddb5dc487a719699f5f
https://pubmed.ncbi.nlm.nih.gov/8170967

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A variant of t-PA (T103N, KHRR 296-299 AAAA) that, by bolus, has increased potency and decreased systemic activation of plasminogen

Autor: C J, Refino, N F, Paoni, B A, Keyt, C S, Pater, J M, Badillo, F M, Wurm, J, Ogez, W F, Bennett

Publikováno v: Thrombosis and haemostasis. 70(2)

In the accompanying paper, we reported that the properties of decreased plasma clearance rate, increased fibrin specificity, and resistance to inactivation by PAI-1 could be effectively combined in the t-PA variant T103N, KHRR 296-299 AAAA. In the cu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9c6b192b846722a41468d06a77518873
https://pubmed.ncbi.nlm.nih.gov/8236141

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A slow clearing, fibrin-specific, PAI-1 resistant variant of t-PA (T103N, KHRR 296-299 AAAA)

Autor: N F, Paoni, B A, Keyt, C J, Refino, A M, Chow, H V, Nguyen, L T, Berleau, J, Badillo, L C, Peña, K, Brady, F M, Wurm

Publikováno v: Thrombosis and haemostasis. 70(2)

Site directed mutagenesis was used to construct a t-PA variant that contains an additional glycosylation site in the first kringle domain (T103N) combined with a tetra-alanine substitution in the protease domain (KHRR 296-299 AAAA). This combination

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e25aff08b11cbf1f52f1f4fbe64f769f
https://pubmed.ncbi.nlm.nih.gov/8236140

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High resolution analysis of functional determinants on human tissue-type plasminogen activator

Autor: W F, Bennett, N F, Paoni, B A, Keyt, D, Botstein, A J, Jones, L, Presta, F M, Wurm, M J, Zoller

Publikováno v: The Journal of biological chemistry. 266(8)

Sixty-four variants of human tissue-type plasminogen activator (tPA) were produced using recombinant DNA techniques. Charged residues were converted to alanine in clusters of from one to four changes per variant; these clusters spanned all the domain

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::69549e899e69494ef3365efaaf05f873
https://pubmed.ncbi.nlm.nih.gov/1900516

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Isolation and characterization of native and lower molecular weight forms of sheep plasminogen

Autor: N F, Paoni, B N, Violand, F J, Castellino

Publikováno v: The Journal of biological chemistry. 252(21)

Two major forms of native sheep plasminogen (SPg-a) have been isolated from plasma by affinity chromatography. These forms differ in molecular weight, charge characteristics, affinity for epsilon-aminocaproic acid (epsilon-Ahx), and carbohydrate cont

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1ae716679ece9ab3000ae41ce2aeadff
https://pubmed.ncbi.nlm.nih.gov/144130

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Improved method for detection of glycosidases in bacterial colonies

Autor: R L Arroyo, N F Paoni

An assay has been developed to detect bacterial glycosidases in colonies grown on the surface of agar plates. Advantages of this technique over previously described methods include elimination of the need for replica plating, better visualization of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8940d7b241b5d829a46962f31180fe1b
https://europepmc.org/articles/PMC239639/

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