Cloning, preparation and characterization of biologically active recombinant caprine placental lactogen

Autor: C Bignon, Edna Sakal, Arieh Gertler, Jean Djiane, N Daniel, J Paly, N Chapnik-Cohen, L Belair

Publikováno v: Journal of Endocrinology
Journal of Endocrinology, BioScientifica, 1998, 159 (3), pp.509-518. ⟨10.1677/joe.0.1590509⟩
Journal of Endocrinology 3 (159), 509-518. (1998)

International audience; Caprine placental lactogen (cPL) cDNA was cloned by reverse transcription (RT)-PCR from total RNA of goat placenta. The PCR product encoding for the mature protein was gel purified, ligated to pGEM-T and finally subcloned into

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::732888ce4994543e4fa52c89be2a5e82
https://doi.org/10.1677/joe.0.1590509

Interaction of recombinant rat placental lactogen-I with extracellular domains of prolactin receptors from three species

Autor: E, Sakal, M C, Robertson, N, Chapnik-Cohen, A, Tchelet, A, Gertler

Publikováno v: Receptorssignal transduction. 6(1)

Rat placental lactogen-I (rPL-I), expressed in rat uteri at midpregnancy, belongs to the GH/PRL/cytokine family of hormones (Wallis, 1992), all members of which exhibit a similar mechanism of receptor activation. Lactogenic activity of rPL-I as deter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2788f872d921c841ba00eab0c58b86dd
https://pubmed.ncbi.nlm.nih.gov/8933620

Preparation of the extracellular domain of the rabbit prolactin receptor expressed in Escherichia coli and its interaction with lactogenic hormones

Autor: A Gertler, E Sakal, Christophe Bignon, N Chapnik-Cohen, Jean Djiane, L Belair

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1994, 269 (5), pp.3318-3324

The cDNA of the extracellular domain of the rabbit prolactin receptor (rbPRLR-ECD) was cloned in the prokaryotic expression vector pTrc99A to enable its expression in Escherichia coli after induction with isopropyl-1-thio-beta-D-galactopyranoside. Th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0df413bafda11f086a2f19fe897506f5
https://hal.inrae.fr/hal-02712883

Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.

Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.