Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Néstor J. Oviedo"'
Autor:
Paul G. Barghouth, Néstor J. Oviedo
Publikováno v:
STAR Protocols, Vol 2, Iss 1, Pp 100257- (2021)
Summary: Whole planarian chromosome squash allows researchers to qualitatively analyze chromosome integrity. Treatment with colchicine is used to halt dividing cells within metaphase and does not require amputation or tissue puncturing. In combinatio
Externí odkaz:
https://doaj.org/article/f2e4b736e54749ef857f39be78c43751
Autor:
Benjamin Ziman, Néstor J. Oviedo
Publikováno v:
STAR Protocols, Vol 2, Iss 1, Pp 100274- (2021)
Summary: In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in
Externí odkaz:
https://doaj.org/article/a479c744a9b146b797ce85fd30b7bf7e
Publikováno v:
Frontiers in Microbiology, Vol 11 (2021)
Candida albicans is one of the most common fungal pathogens of humans. Prior work introduced the planarian Schmidtea mediterranea as a new model system to study the host response to fungal infection at the organismal level. In the current study, we a
Externí odkaz:
https://doaj.org/article/c0b2e66f606d47d682dd3c0912955529
Publikováno v:
iScience, Vol 23, Iss 11, Pp 101665- (2020)
Summary: Tissue homeostasis relies on the timely renewal of cells that have been damaged or have surpassed their biological age. Nonetheless, the underlying molecular mechanism coordinating tissue renewal is unknown. The planarian Schmidtea mediterra
Externí odkaz:
https://doaj.org/article/c234c68334604b8f85f796ee8ed3911a
Autor:
Kamal S. Dulai, Petra Kranzfelder, Adriana Signorini, Téa S. Pusey, Andrea Presas Valencia, Christian Urbina, Néstor J. Oviedo
Publikováno v:
CBE—Life Sciences Education. 21
Student-centered pedagogies promote student learning in college science, technology, engineering, and mathematics (STEM) classrooms. However, transitioning to active learning from traditional lecturing may be challenging for both students and instruc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6bea15efdc375097e87b31d1022c4458
https://doi.org/10.1187/cbe.21-08-0199
https://doi.org/10.1187/cbe.21-08-0199
Publikováno v:
Bioelectricity
Background: The use of direct current electric stimulation (DCS) is an effective strategy to treat disease and enhance body functionality. Thus, treatment with DCS is an attractive biomedical alternative, but the molecular underpinnings remain mostly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f9d85f87ce87ec4291468ab7e8af352
https://doi.org/10.1089/bioe.2020.0026
https://doi.org/10.1089/bioe.2020.0026
Autor:
Devon Davidian, Melanie LeGro, Paul G. Barghouth, Salvador Rojas, Benjamin Ziman, Eli Isael Maciel, David Ardell, Ariel L. Escobar, Néstor J. Oviedo
Publikováno v:
J Cell Sci
Exposure to high levels of ionizing γ radiation leads to irreversible DNA damage and cell death. Here, we establish that exogenous application of electric stimulation enables cellular plasticity and the re-establishment of stem cell activity in tiss
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68955874da5f0d19c73cfe4cdb8731fe
https://europepmc.org/articles/PMC9264365/
https://europepmc.org/articles/PMC9264365/
Publikováno v:
Development
Immune cells are known to engage in pathogen defense. However, emerging research has revealed additional roles for immune cells, which are independent of their function in the immune response. Here, we underscore the ability of cells outside of the a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bd833b10f26e00ccae701013d659a11
https://doi.org/10.1242/dev.199903
https://doi.org/10.1242/dev.199903
Publikováno v:
Methods Mol Biol
Methods in Molecular Biology ISBN: 9781071621714
Methods in Molecular Biology ISBN: 9781071621714
Comet assay provides the opportunity to detect and characterize DNA strand breaks. Cellular lysing followed by embedding in agarose slide is used to visualize under an electrical current migration patterns corresponding to DNA fragments of different
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39e55f966c23a0e051e39a745a4b9c53
https://europepmc.org/articles/PMC9761910/
https://europepmc.org/articles/PMC9761910/
Autor:
Ziman B, Maciel Ei, Davidian D, Ardell Dh, Barghouth Pg, Néstor J. Oviedo, LeGro M, Escobar Al, Rojas S
Exposure to high levels of ionizing γ-radiation leads to irreversible DNA damage and cell death. Here, we establish that exogenous application of electric stimulation enables cellular plasticity to reestablish stem cell activity in tissues damaged b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4a518df15c0691a500ea1c1c945bf640
https://doi.org/10.1101/2021.05.16.444365
https://doi.org/10.1101/2021.05.16.444365