Zobrazeno 1 - 10
of 431
pro vyhledávání: '"N, Brot"'
Publikováno v:
The Journal of Immunology. 146:3386-3395
Considerable evidence supports the idea that autoantibody production in human and murine SLE is Ag driven. To determine whether Ag (the ribosomal P proteins) could initiate autoantibody production in lupus mice, 34 MRL/lpr mice were immunized with mo
Publikováno v:
Journal of bacteriology. 177(5)
In Escherichia coli, the growth rate at elevated temperatures is controlled by the availability of endogenous methionine, which is limited because of the temperature sensitivity of the metA gene product, homoserine transsuccinylase (HTS). In order to
Publikováno v:
Methods in enzymology. 251
Publikováno v:
Cellularmolecular biology research. 41(5)
A rapid and simple spin column assay has been used to study interactions of BiP with substance P (SP) and ATP. At 4 degrees C, the binding of SP to BiP requires ATP and a stable SP-BiP.ATP complex is formed. Nonhydrolyzable ATP analogues or ADP canno
Publikováno v:
Advances in experimental medicine and biology. 347
ARA occur in approximately 10% of randomly selected SLE patients but in up to 40% of patients with active disease. Anti-P antibodies appear to be a highly specific diagnostic marker for SLE since they are rarely detected in other multisystem autoimmu
High level expression and purification of peptide methionine sulfoxide reductase in Escherichia coli
Publikováno v:
Cellular and molecular biology. 38(5)
The enzyme peptide methionine sulfoxide reductase catalyzes the conversion of methionine sulfoxide residues in proteins to methionine. The 636 nucleotide coding region of the peptide methionine sulfoxide reductase gene has been amplified from a genom
Publikováno v:
Cellular and molecular biology. 38(1)
Several members of the 70 kDa heat shock protein group are known to be phosphorylated in vivo and have recently been found to undergo a Ca(2+)-stimulated autophosphorylation. The characteristics of the autophosphorylation reaction with Escherichia co
Autor:
N, Brot, H, Weissbach
Publikováno v:
BioFactors (Oxford, England). 3(2)
The oxidation of methionine to methionine sulfoxide constitutes one of the many post-translational modifications that proteins undergo. This non-enzymatic reaction has been shown to occur both in vivo and in vitro, and has been associated with the lo
Publikováno v:
Biochemical and Biophysical Research Communications. 69:608-612
The uptake of radioactive ribosomal proteins by isolated HeLa cell nuclei has been studied. Ribosomal proteins are taken up by nuclei in vitro more rapidly than are cytosol proteins, suggesting that the uptake is selective. In addition, the ribosomal
Publikováno v:
The Journal of Immunology. 140:3434-3437
Autoantibodies reactive against a shared, conserved epitope on the ribosomal phosphoproteins P0, P1, and P2 occur in approximately 15% of patients with SLE and are relatively specific for this disease. To determine whether anti-P antibodies occur in