Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Myra H, Robinson"'
Publikováno v:
ELECTROPHORESIS. 25:2511-2519
The fluorescence of proteins stained with Deep Purple and SYPRO Ruby was measured over a time course of UV transillumination to determine the relative photostability of each stain. Mean spot fluorescence (n = 200 matched spots) in gels stained with D
Autor:
Elena Chernokalskaya, Kiera Berggren, Myra H. Robinson, Wayne F. Patton, Alexander Lazarev, Mary F. Lopez
Publikováno v:
Electrophoresis. 21:3673-3683
Proteomic projects are often focused on the discovery of differentially expressed proteins between control and experimental samples. Most laboratories choose the approach of running two-dimensional (2-D) gels, analyzing them and identifying the diffe
Autor:
Elena Chernokalskaya, Myra H. Robinson, Alla Bogdanova, Mary F. Lopez, Bruce S. Kristal, Alexander Lazarev, Alex I. Shestopalov
Publikováno v:
Electrophoresis. 21:3427-3440
Recent studies have demonstrated the need for complementing cellular genomic information with specific information on expressed proteins, or proteomics, since the correlation between the two is poor. Typically, proteomic information is gathered by an
Autor:
Myra H. Robinson, Gary B. Smejkal
Publikováno v:
Electrophoresis. 28(10)
When dried IPGs are hydrated with protein solutions, the concentration of protein and other ionic constituents is constant throughout the strip. Tris, initially present at a very low concentration, focuses during IEF and accumulates in the gradient a
Autor:
Gary B, Smejkal, Myra H, Robinson, Nathan P, Lawrence, Feng, Tao, Calvin A, Saravis, Richard T, Schumacher
Publikováno v:
Journal of biomolecular techniques : JBT. 17(2)
Sample preparation is critical to the success of two-dimensional gel electrophoresis and other analytical methods. Pressure-cycling technology (PCT) uses alternating cycles of high and low pressure to induce cell lysis. Cell suspensions were placed i
Autor:
Myra H. Robinson, Chunqin Li, Elena Chernokalskaya, Alexander Lazarev, Gary B. Smejkal, Nathan Lawrence
Publikováno v:
Journal of proteome research. 5(4)
Reduction and alkylation of protein disulfides prior to IEF, when performed directly in a centrifugal ultrafiltration device, provides an effective means of terminating the alkylation reaction, concentrating the proteins for analysis, and removing io