Zobrazeno 1 - 10
of 109
pro vyhledávání: '"Mutsumi Takagi"'
Publikováno v:
Cytotechnology. 74:193-200
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::838a49d1d5b031cea3a877dea5973a22
https://doi.org/10.1007/s10616-021-00505-z
https://doi.org/10.1007/s10616-021-00505-z
Publikováno v:
Journal of Artificial Organs. 23:358-364
The cultivation of cells on microcarriers (MCs) in stirred suspension system is useful method for the large-scale culture of human mesenchymal stem cells (hMSCs) for allogenic transplantation. To harvest hMSCs from MCs without using proteolytic enzym
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a850dfd75544c0657d4467e5238b20d
https://doi.org/10.1007/s10047-020-01186-9
https://doi.org/10.1007/s10047-020-01186-9
Publikováno v:
Cytotechnology
Although nonwoven fabric (NWF) has been reported to be a candidate scaffold for the large-scale expansion of mesenchymal stem cells (MSCs), the quality of cells grown in NWF has not been well clarified. In this report, MSCs grown in an NWF disc for 3
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ba387f0e87917067baecec435844e62
https://doi.org/10.1007/s10616-020-00390-y
https://doi.org/10.1007/s10616-020-00390-y
Publikováno v:
Journal of bioscience and bioengineering. 131(2)
While continuous (perfusion) culture of mammalian cells might reduce the reactor size owing to the high cell density, there is the problem of higher medium cost; however, this problem is expected to be solved by the reuse of growth-promoting componen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a6a60ca8176632000e526737838db7e
https://pubmed.ncbi.nlm.nih.gov/33051156
https://pubmed.ncbi.nlm.nih.gov/33051156
Publikováno v:
Cytotechnology
To obtain a large number of human mesenchymal stem cell (hMSCs) for allograft, nonwoven fabrics (NWF) were used as a cell culture scaffold. NWF are three-dimensional fiber aggregates formed by heat bonding and have a high surface area for cell adhesi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67e1d7d344373bf7a88156c3462fb4be
https://europepmc.org/articles/PMC6546790/
https://europepmc.org/articles/PMC6546790/
Autor:
Hirotaka Wada, Hideki Matsumoto, Mutsumi Takagiwa, Hitomi Sato, Kyoko Ishiguchi, Aya Inoue, Tazuko K. Goto
Publikováno v:
Frontiers in Nutrition, Vol 11 (2024)
BackgroundTo understand age-related changes in sweet taste perception in daily life, it is important to understand taste intensity at the suprathreshold level. Previous studies have attempted to characterize the temporal aspects of human taste percep
Externí odkaz:
https://doaj.org/article/c6663c33c77141828139a77e81572d05
Autor:
Naohide Tomita, Mutsumi Takagi, Nobuhiro Kasai, Yusuke Morita, Shigeyuki Wakitani, Hisashi Mera
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 81:197-199
The effects of epigallocatechin-3-o-gallate (EGCG) and quercetin on the contents of extracellular matrix (ECM) in porcine cartilage at 4 °C were investigated. The addition of quercetin at 0.01 mM for the incubation of porcine cartilage disks at 4 °
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::204413aaa92ec805dfc646091963ceb2
https://doi.org/10.1080/09168451.2016.1232156
https://doi.org/10.1080/09168451.2016.1232156
Autor:
Daisuke Takahashi, Hisashi Mera, Norimasa Iwasaki, Mutsumi Takagi, Tokifumi Majima, Yasushi Sato, Shigeyuki Wakitani
Publikováno v:
Cytotechnology. 69:405-416
Aiming to increase the content of type 2 collagen in scaffold-free cartilage-like cell sheets prepared using human bone marrow mesenchymal stem cells, the effect of several kinds of additives in a chondrogenic medium was investigated. Addition of asc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a97ced9a2dbf93da52c77073a46dfd3
https://doi.org/10.1007/s10616-015-9924-3
https://doi.org/10.1007/s10616-015-9924-3
Publikováno v:
Bioscience, biotechnology, and biochemistry. 81(6)
With the aim to increase type II collagen content in the scaffold-free cartilage-like cell sheet using human bone marrow mesenchymal stem cells, we examined the effect of epigallocatechin-3-gallate (EGCG) addition to the chondrogenic medium for the c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b6bacb74c2b145d305f78b1b8ca8c9a
https://pubmed.ncbi.nlm.nih.gov/28485205
https://pubmed.ncbi.nlm.nih.gov/28485205
Autor:
Mutsumi Takagi, Hideo Miki
Publikováno v:
Cytotechnology. 67:689-697
The design of serum-free media for suspension culture of genetically engineered Chinese hamster ovary (CHO) cells using general commercial media as a basis was investigated. Subcultivation using a commercial serum-free medium containing insulin-like
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c36b75595c33da8e442f8981dd84db2
https://doi.org/10.1007/s10616-014-9778-0
https://doi.org/10.1007/s10616-014-9778-0