Enantioselective Synthesis of Pharmaceutically Active γ-Aminobutyric Acids Using a Tailor-Made Artificial Michaelase in One-Pot Cascade Reactions

Autor: Pieter G. Tepper, Gerrit J. Poelarends, Simon J. Charnock, Saravanan Thangavelu, Ronald van Merkerk, Lieuwe Biewenga, Tjaard Pijning, Jan-Ytzen van der Meer, Andy-Mark W. H. Thunnissen, Andreas Kunzendorf

Publikováno v: ACS Catalysis, 9, 1503-1513. AMER CHEMICAL SOC
ACS Catalysis
ACS catalysis 9(2), 1503-1513 (2019). doi:10.1021/acscatal.8b04299

Chiral γ-aminobutyric acid (GABA) analogues represent abundantly prescribed drugs, which are broadly applied as anticonvulsants, as antidepressants, and for the treatment of neuropathic pain. Here we report a one-pot two-step biocatalytic cascade ro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3f4789471bf3ac24adfd77a804ba91c
https://doi.org/10.1021/acscatal.8b04299

P450-Catalyzed Regio- and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping

Autor: Richard Lonsdale, Kirsty J. McLean, Charles G. Gamble, Yuval Nov, Christophe Farès, Aitao Li, Julia B. Lingnau, Adrian J. Mulholland, Manfred T. Reetz, David Leys, Heike Hinrichs, Alfred Deege, Sabrina Hoebenreich, Carlos G. Acevedo-Rocha, Nathalie Nett, Cornelia Wirtz, Andrew W. Munro

Publikováno v: Acevedo-Rocha, C G, Gamble, C, Lonsdale, R, Li, A, Nett, N, Hoebenreich, S, Lingnau, J B, Wirtz, C, Fares, C, Hinrichs, H, Deege, A, Mulholland, A J, Nov, Y, Leys, D, Mclean, K, Munro, A & Reetz, M T 2018, ' P450-Catalyzed Regio-and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping ', ACS Catalysis, vol. 8, no. 4, pp. 3395-3410 . https://doi.org/10.1021/acscatal.8b00389

Cytochrome P450 monooxygenases play a crucial role in the biosynthesis of many natural products and in the human metabolism of numerous pharmaceuticals. This has inspired synthetic organic and medicinal chemists to exploit them as catalysts in regio-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::376c458d57db555de36b041a9eb1e1bb
https://www.research.manchester.ac.uk/portal/en/publications/p450catalyzed-regio-and-diastereoselective-steroid-hydroxylation-efficient-directed-evolution-enabled-by-mutability-landscaping(e831d47c-8f3f-42d1-b78c-5a2e390dc964).html

Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy

Autor: Aitao Li, Carlos G. Acevedo-Rocha, Manfred T. Reetz

Publikováno v: Applied Microbiology and Biotechnology

Site-saturation mutagenesis (SSM) has been used in directed evolution of proteins for a long time. As a special form of saturation mutagenesis, it involves individual randomization at a given residue with formation of all 19 amino acids. To date, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04045f0d755c380f1b28eee91a10236a
https://pubmed.ncbi.nlm.nih.gov/29785500

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