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Kinetics of mRNA and protein synthesis of genes controlled by the 1,4- β-endoxylanase A promoter in controlled fermentations of Aspergillus awamori

Autor: Gouka, R.J., Stam, H., Fellinger, A.J., Muijsenberg, R.J.G.T., Wijngaard, A.J. van de, Punt, P.J., Musters, W., Hondel, C.A.M.J.J. van den

Publikováno v: Applied and Environmental Microbiology, 10, 62, 3646-3649

In this study, induction and repression kinetics of the expression of the Aspergillus awamori 1,4-β-endoxylanase A (exlA) gene under defined physiological conditions was analyzed at the mRNA and the protein levels. Induction was analyzed by pulsing

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An expression system based on the promoter region of the Aspergillus awamori 1,4-bèta-endoxylanase A gene

Autor: Gouka, R.J., Hessing, J.G.M., Punt, P.J., Stam, H., Musters, W., Hondel, C.A.M.J.J. van den

Publikováno v: Appied Microbiology and Biotechnology, 46, 28-35

A new, highly inducible fungal promoter derived from the Aspergillus awamori 1,4-β-endoxylanase A (exlA) gene is described. Induction analysis, carried out with the wild-type strain in shake flasks, showed that exlA expression is regulated at the tr

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Cloning and expression of DNA encoding a ripening form of a polypeptide having rhamnogalacturonase activity

Autor: Musters, W., Stam, H., Suykerbuyk, M.E., Visser, J., Verbakel, J.M.

The invention relates to isolation of an Aspergillus gene encoding rhamnogalacturonase (RG-ase) and the construction of recombinant Aspergillus strains with overexpression of RG-ase. These strains can be used for the commercial production of RG-ase.

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https://research.wur.nl/en/publications/cloning-and-expression-of-dna-encoding-a-ripening-form-of-a-polyp

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Cloning and expression of DNA encoding a ripening form a polypeptide having sulfhydryl oxidase activity

Autor: Maat, J., Musters, W., Stam, H., Schaap, P.J., van de Vondervoort, P.J.J., Visser, J., Verbakel, J.M.A.

The invention relates to recombinant DNA technology for the production of an enzyme having sulfhydryl oxidase ("SOX") activity. This SOX-enzyme can be used where the oxidation of free sulfhydryl groups (thio compounds) to the corresponding disulfides

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::52227e4e9548c29ebe0baca8ddf6b29f
https://research.wur.nl/en/publications/cloning-and-expression-of-dna-encoding-a-ripening-form-a-polypept

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