Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Muppalla Sukumar"'
Publikováno v:
Journal of Pharmaceutical Sciences. 101:519-529
Surface adsorption of two monoclonal antibodies (mAb1 and mAb2), with widely different hydrophobicity and self-association behavior in solution, was examined by quartz crystal microbalance with dissipation monitoring to understand how adsorption and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e232bac8c1a458aff9e379b55504f8b
https://doi.org/10.1002/jps.22771
https://doi.org/10.1002/jps.22771
Autor:
Muppalla Sukumar, Peter M. Tessier, Anna Marie Marcelino-Cruz, Shantanu Sule, William F. Weiss, Tyler Sample
Publikováno v:
Biophysical Journal. 101(7):1749-1757
Monoclonal antibodies are typically monomeric and nonviscous at low concentrations, yet they display highly variable associative and viscous behavior at elevated concentrations. Although measurements of antibody self-association are critical for unde
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7204a7d528880cccee4b8e4bc2a5102
Publikováno v:
Pharmaceutical Research. 22:789-796
Manufacturing processes expose protein pharmaceuticals to organic solvents that may perturb the native folded state, increasing the potential for irreversible aggregation or surface adsorption. The aim of this study was to characterize the conformati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a682873d814e78b72f04afd64eee0c2f
https://doi.org/10.1007/s11095-005-2596-5
https://doi.org/10.1007/s11095-005-2596-5
Publikováno v:
Pharmaceutical Research. 21:1087-1093
Purpose. Therapeutic antibodies are often formulated at a high concentration where they may have an opalescent appearance. The aim of this study is to understand the origin of this opalescence, especially its relationship to noncovalent association a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea08f7d931cbf1a389d4fca0c5347196
https://doi.org/10.1023/b:pham.0000032993.98705.73
https://doi.org/10.1023/b:pham.0000032993.98705.73
Autor:
Amy L. Cox, Ronald E. Chance, David S. McClellan, Sharon R. Myers, Mark L. Brader, Allen H. Pekar, David B. Flora, Lynnie A. Irwin, Muppalla Sukumar
Publikováno v:
Nature Biotechnology. 20:800-804
The ability to tailor the release profile of a drug by manipulating its formulation matrix offers important therapeutic advantages. We show here that human insulin can be cocrystallized at preselected ratios with the fully active lipophilically modif
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8790eb3ca0b2a121ab35819f7e94f75a
https://doi.org/10.1038/nbt722
https://doi.org/10.1038/nbt722
Publikováno v:
Biochemistry. 39:9119-9129
Cellular retinoic acid binding protein I (CRABPI) belongs to the family of intracellular lipid binding proteins (iLBPs), all of which bind a hydrophobic ligand within an internal cavity. The structures of several iLBPs reveal minimal structural diffe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5fc4c2ab3b8fc856f002233d7718d70
https://doi.org/10.1021/bi000296l
https://doi.org/10.1021/bi000296l
Publikováno v:
Journal of pharmaceutical sciences. 99(6)
Micro-flow imaging (MFI) has been shown to be more sensitive than light obscuration (LO) methods for measuring subvisible proteinaceous particles in protein formulations. Given the potential challenges in detecting particulates in opalescent mAb form
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ba8f887111538ce1bb5d64025a47b59
https://pubmed.ncbi.nlm.nih.gov/20049937
https://pubmed.ncbi.nlm.nih.gov/20049937
Autor:
Allen H. Pekar, Muppalla Sukumar
Publikováno v:
Analytical biochemistry. 367(2)
Aggregation is a major degradation pathway that needs to be characterized and controlled during the development of protein pharmaceuticals. Analytical ultracentrifugation-sedimentation velocity (AUC-SV) is emerging as an important orthogonal tool to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43679d3fb7c2c10e5480567d860062f1
https://pubmed.ncbi.nlm.nih.gov/17548043
https://pubmed.ncbi.nlm.nih.gov/17548043
Autor:
Kenneth S. Rotondi, B.F. Weston, Muppalla Sukumar, P. L. Clark, Lila M. Gierasch, Zhi Ping Liu, Josep Rizo
Publikováno v:
American Peptide Symposia ISBN: 0792351606
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1e21b6d07869f3c8a2f89c8ce29ff9e1
https://doi.org/10.1007/0-306-46862-x_150
https://doi.org/10.1007/0-306-46862-x_150
