Super-resolution correlative light-electron microscopy using a click-chemistry approach for studying intracellular trafficking

Autor: Andrian, T., Bakkum, T., Elsland, D.M. van, Bos, E., Koster, A.J., Albertazzi, L., Kasteren, S.I. van, Pujals, S., MullerReichert, T., Verkade, P.

Publikováno v: Methods in Cell Biology, 162, 303-331. Elsevier
Methods in Cell Biology
Methods in Cell Biology-Correlative Light and Electron Microscopy IV
Correlative Light and Electron Microscopy IV, 303-331
STARTPAGE=303;ENDPAGE=331;TITLE=Correlative Light and Electron Microscopy IV
Methods in Cell Biology, 162, 303-331
Correlative Light and Electron Microscopy IV
Methods in Cell Biology ISBN: 9780128220580

Correlative light and electron microscopy (CLEM) entails a group of multimodal imaging techniques that are combined to pinpoint to the location of fluorescently labeled molecules in the context of their ultrastructural cellular environment. Here we d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b55f38c62c043116890b4183d72225e
https://hdl.handle.net/1887/3157127

Correlated Light Microscopy and Electron Microscopy

Autor: Sjollema, Klaas A., Schnell, Ulrike, Kuipers, Jeroen, Kalicharan, Ruby, Giepmans, Ben N. G., MullerReichert, T, Verkade, P

Publikováno v: CORRELATIVE LIGHT AND ELECTRON MICROSCOPY, 157-173
STARTPAGE=157;ENDPAGE=173;TITLE=CORRELATIVE LIGHT AND ELECTRON MICROSCOPY

Understanding where, when, and how biomolecules (inter)act is crucial to uncover fundamental mechanisms in cell biology. Recent developments in fluorescence light microscopy (FLM) allow protein imaging in living cells and at the near molecular level.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d30f83ea2146cfb44f3ff23034c6f61
https://doi.org/10.1016/b978-0-12-416026-2.00009-1

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CPAP is a cell-cycle regulated protein that controls centriole length.

Autor: Tang, Chieh-Ju C.¹, Fu, Ru-Huei^1,2, Wu, Kuo-Sheng^1,3, Hsu, Wen-Bin¹, Tang, Tang K.¹ tktang@ibms.sinica.edu.tw

Publikováno v: Nature Cell Biology. Jul2009, Vol. 11 Issue 7, p825-831. 7p. 4 Diagrams, 9 Graphs.

Correlative In Vivo 2-Photon Beam Scanning Electron Microscopy: 3D Analysis of Neuronal Ultrastructure

Autor: Maco, Bohumil, Holtmaat, Anthony, Jorstad, Anne, Fua, Pascal, Knott, Graham

This protocol describes how dendrites and axons, imaged in vivo, can subsequently be analyzed in 3D using focused ion beam scanning electron microscopy (FIBSEM). The fluorescent structures are identified after chemical fixation and their position hig

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______185::f96132aa8127b6686ede88e0a36d8e28
https://infoscience.epfl.ch/record/203945