Robust estimation of bacterial cell count from optical density

Autor: Tyler Chapman, Alfonso Jaramillo, Burhan Sabuwala, Sathvik Anantakrishnan, Rishi Rajendra Gandhi, Dominic Schwarz, Clarissa Melo Czekster, Simon Powis, Geoffrey Baldwin, Francisco Zorrilla, Darshak Bhatt, Jacob Beal, Luc Roberts, Guillem Lopez-Grado i Salinas, Kristi Turton, Diptatanu Das, Serena Petracchini, Natalie Farny, Christopher Workman, Stephanie Heux, Ana Luisa Mendes dos Santos, Muhammad Iqbal Adi Pratama, Ioannis Kostopoulos, Kailash B P

Publikováno v: Communications Biology
Interlab Study Contributors iGEM, Beal, J, Farny, N G, Haddock-Angelli, T, Selvarajah, V, Baldwin, G S, Buckley-Taylor, R, Gershater, M, Kiga, D, Marken, J, Sanchania, V, Sison, A & Workman, C T 2020, ' Robust estimation of bacterial cell count from optical density ', Communications Biology, vol. 3, no. 1, 512 . https://doi.org/10.1038/s42003-020-01127-5
Communications Biology, Vol 3, Iss 1, Pp 1-29 (2020)

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an

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