Production of trimeric SARS‐CoV‐2 spike protein by CHO cells for serological COVID‐19 testing

Autor: Johari, Y.B., Jaffé, S.R.P., Scarrott, J.M., Johnson, A.O., Mozzanino, T., Pohle, T.H., Maisuria, S., Bhayat‐Cammack, A., Lambiase, G., Brown, A.J., Tee, K.L., Jackson, P.J., Wong, T.S., Dickman, M.J., Sargur, R.B., James, D.C.

We describe scalable and cost‐efficient production of full length, His‐tagged severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) spike glycoprotein trimer by Chinese hamster ovary (CHO) cells that can be used to detect SARS‐CoV‐

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https://eprints.whiterose.ac.uk/171845/1/bit.27615.pdf

Production of trimeric SARS-CoV-2 spike protein by CHO cells for serological COVID-19 testing.

Autor: Johari YB; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Jaffé SRP; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Scarrott JM; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Johnson AO; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Mozzanino T; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Pohle TH; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Maisuria S; Department of Immunology, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., Bhayat-Cammack A; Department of Immunology, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., Lambiase G; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Brown AJ; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Tee KL; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Jackson PJ; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Wong TS; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Dickman MJ; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK., Sargur RB; Department of Immunology, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK., James DC; Department of Chemical and Biological Engineering, University of Sheffield, Mappin St., Sheffield, UK.

Publikováno v: Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Feb; Vol. 118 (2), pp. 1013-1021. Date of Electronic Publication: 2020 Nov 11.