Characterization of Higher Order Structural Changes of a Thermally Stressed Monoclonal Antibody via Mass Spectrometry Footprinting and Other Biophysical Approaches.

Autor: Lin Y; Department of Chemistry, Washington University in St Louis, St Louis, Missouri 63105, United States., Moyle AB; Department of Chemistry, Washington University in St Louis, St Louis, Missouri 63105, United States., Beaumont VA; Pharmaceutical Sciences Small Molecules, Analytical Research and Development, Pfizer, Inc., Sandwich CT13 9FF, U.K., Liu LL; Biotherapeutics Pharmaceutical Sciences, Analytical Research and Development, Pfizer, Inc., Andover, Massachusetts 01810, United States., Polleck S; Biotherapeutics Pharmaceutical Sciences, Analytical Research and Development, Pfizer, Inc., Andover, Massachusetts 01810, United States., Liu H; Department of Chemistry, Washington University in St Louis, St Louis, Missouri 63105, United States., Shi H; Global Product Development, Rare Disease Statistics, Pfizer, Inc., New York, New York 10017, United States., Rouse JC; Biotherapeutics Pharmaceutical Sciences, Analytical Research and Development, Pfizer, Inc., Andover, Massachusetts 01810, United States., Kim HY; Biotherapeutics Pharmaceutical Sciences, Analytical Research and Development, Pfizer, Inc., Andover, Massachusetts 01810, United States., Zhang Y; Biotherapeutics Pharmaceutical Sciences, Analytical Research and Development, Pfizer, Inc., Andover, Massachusetts 01810, United States., Gross ML; Department of Chemistry, Washington University in St Louis, St Louis, Missouri 63105, United States.

Publikováno v: Analytical chemistry [Anal Chem] 2023 Nov 21; Vol. 95 (46), pp. 16840-16849. Date of Electronic Publication: 2023 Nov 07.

Workflow for Validating Specific Amino Acid Footprinting Reagents for Protein Higher Order Structure Elucidation.

Autor: Moyle AB; Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Wagner ND; Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Wagner WJ; Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Cheng M; Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Gross ML; Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States.

Publikováno v: Analytical chemistry [Anal Chem] 2023 Jul 04; Vol. 95 (26), pp. 10119-10126. Date of Electronic Publication: 2023 Jun 23.

Benzoyl Transfer for Footprinting Alcohol-Containing Residues in Higher Order Structural Applications of Mass-Spectrometry-Based Proteomics.

Autor: Moyle AB; Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Cheng M; Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Wagner ND; Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Gross ML; Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri 63130, United States.

Publikováno v: Analytical chemistry [Anal Chem] 2022 Jan 25; Vol. 94 (3), pp. 1520-1524. Date of Electronic Publication: 2022 Jan 12.

Lrp1 is a host entry factor for Rift Valley fever virus.

Autor: Ganaie SS; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Schwarz MM; Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA; Department of Infectious Diseases and Microbiology, School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA., McMillen CM; Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA; Department of Infectious Diseases and Microbiology, School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA., Price DA; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Feng AX; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Albe JR; Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA., Wang W; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Miersch S; The Donnelly Centre, University of Toronto, Toronto, ON, Canada., Orvedahl A; Department of Pediatrics, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Cole AR; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Sentmanat MF; Genome Engineering and iPSC Center (GEiC), Department of Genetics, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Mishra N; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Boyles DA; Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA., Koenig ZT; Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA; Department of Infectious Diseases and Microbiology, School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA., Kujawa MR; Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA; Department of Infectious Diseases and Microbiology, School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA., Demers MA; Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA., Hoehl RM; Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA., Moyle AB; Department of Chemistry, Washington University in St. Louis, St. Louis, MO, USA., Wagner ND; Department of Chemistry, Washington University in St. Louis, St. Louis, MO, USA., Stubbs SH; Department of Microbiology, Harvard Medical School, Boston, MA, USA., Cardarelli L; The Donnelly Centre, University of Toronto, Toronto, ON, Canada., Teyra J; The Donnelly Centre, University of Toronto, Toronto, ON, Canada., McElroy A; Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA; Department of Pediatrics, Division of Pediatric Infectious Disease, University of Pittsburgh, Pittsburgh, PA, USA., Gross ML; Department of Chemistry, Washington University in St. Louis, St. Louis, MO, USA., Whelan SPJ; Department of Molecular Microbiology, Washington University in St. Louis, St. Louis, MO, USA., Doench J; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Cui X; Genome Engineering and iPSC Center (GEiC), Department of Genetics, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Brett TJ; Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Sidhu SS; The Donnelly Centre, University of Toronto, Toronto, ON, Canada., Virgin HW; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA; Current address: Vir Biotechnology, San Francisco, CA, USA., Egawa T; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Leung DW; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA; Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Amarasinghe GK; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA. Electronic address: gamarasinghe@wustl.edu., Hartman AL; Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA; Department of Infectious Diseases and Microbiology, School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: hartman2@pitt.edu.

Publikováno v: Cell [Cell] 2021 Sep 30; Vol. 184 (20), pp. 5163-5178.e24. Date of Electronic Publication: 2021 Sep 23.

Characterization of SARS-CoV-2 N protein reveals multiple functional consequences of the C-terminal domain.

Autor: Wu C; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Qavi AJ; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Hachim A; HKU-Pasteur Research Pole, School of Public Health, The University of Hong Kong, Hong Kong, China., Kavian N; HKU-Pasteur Research Pole, School of Public Health, The University of Hong Kong, Hong Kong, China.; Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Assistance Publique-Hôpitaux de Paris, Hôpital Universitaire Paris Centre, Centre Hospitalier Universitaire Cochin, Service d'Immunologie Biologique, Paris, France.; Institut Cochin, INSERM U1016, Université Paris Descartes, Sorbonne Paris Cité, Paris., Cole AR; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Moyle AB; Department of Chemistry, Washington University in St. Louis, St. Louis, MO, USA., Wagner ND; Department of Chemistry, Washington University in St. Louis, St. Louis, MO, USA., Sweeney-Gibbons J; Center for Microbial Pathogenesis, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA., Rohrs HW; Department of Chemistry, Washington University in St. Louis, St. Louis, MO, USA., Gross ML; Department of Chemistry, Washington University in St. Louis, St. Louis, MO, USA., Peiris JSM; HKU-Pasteur Research Pole, School of Public Health, The University of Hong Kong, Hong Kong, China.; Division of Public Health Laboratory Sciences, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China., Basler CF; Center for Microbial Pathogenesis, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA., Farnsworth CW; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Valkenburg SA; HKU-Pasteur Research Pole, School of Public Health, The University of Hong Kong, Hong Kong, China., Amarasinghe GK; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA., Leung DW; Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.; Department of Internal Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2020 Nov 30. Date of Electronic Publication: 2020 Nov 30.