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pro vyhledávání: '"Motohiko, Hikuma"'
Autor:
Motohiko, Hikuma
Publikováno v:
帝京科学大学紀要 = Bulletin of Teikyo University of Science. 4:31-39
A microbial electrode was constructed with immobilized nitrifying bacteria and an oxygen probe to examine the inhibition of chemicals on respiration. Substrates for ammonia and nitrite oxidizers were introduced into the flow cell of the electrode acc
Publikováno v:
Biochemical Engineering Journal. 15:205-210
Heating treatment as a permeabilization method for plant cells was examined on the release of anthocyanin pigments from strawberry cells. The release rate of anthocyanins followed an Arrhenius-type dependency on temperature. The death rate was observ
Publikováno v:
Biotechnology and Bioprocess Engineering. 7:323-326
To detect whole ammonia-oxidizing bacteria in the activated sludge, group-specific primers targeting the 16S-rRNA gene of ammonia-oxidizing bacteria were used. The electrophoresis pattern of the PCR products seemed to produce a single band of approxi
Autor:
Motohiko Hikuma, Hiroyuki Kobayashi
Publikováno v:
Analytical Letters. 33:1013-1023
Nitrite was reduced in acidic potassium iodide solution to produce nitrogen oxide which was measured amperometrically by a modified gold electrode covered with a PTFE-membrane as the permeability barrier for interfering substances in the sample. The
Publikováno v:
Applied Microbiology and Biotechnology. 44:37-42
The copolyester of 3-hydroxybutyrate and 3- hydroxyvalerate was synthesized from the combined carbon sources of glucose and sodium propionate by a filamentaion-defective mutant of Sphaerotilus natans, which is a typical filamentous bacterium often fo
Publikováno v:
Scopus-Elsevier
The sensor described consists of an anode mounted with immobilized alcohol dehydrogenase from Gluconobacter oxydons, a cathode and an electrolyte containing hexacyanoferrate(III). Its surface was covered with a gas-permeable membrane. Measurements we
Publikováno v:
Applied Microbiology and Biotechnology. 43:31-34
A sheathed bacterium Sphaerotilus natans could not survive at 4°C for 2 months, and mutants that exhibited different colony phenotypes were obtained only by repeating the short period of storage at 4 °C. The ability of these mutants and the parent
Publikováno v:
Biotechnology Techniques. 7:231-236
The microbial electrode consisted of immobilizedPseudomonas aeruginosa (ATCC 10145) and a carbon dioxide gas-sensing probe. The calibration curve was linear from 20 to 200 mg/l of nitrate with a slope of 30 mv/decade. A measurement was made within 10
Publikováno v:
Analytical Letters. 26:209-221
The long-time stability of enzyme electrodes consisting of immobilized enzyme and an oxygen probe was examined at 30°C and 15∼20°C (the sensitivity was 60% of that found at 30°C). The lactate (based on oxidase from Pediococcus sp.) and the alcoh
Publikováno v:
KAGAKU KOGAKU RONBUNSHU. 19:901-907
アンモニア計測用の微生物電極は, 硝化細菌を固定化した微生物膜と酸素電極を組み合わせて作製した.BOD (Biochemical oxygen demand) 計測用の微生物電極は, 硝化細菌に代えて酵母Trichospron cutane