Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Motoaki Wakiyama"'
Autor:
Takayoshi Matsuda, Takuhiro Ito, Chie Takemoto, Kazushige Katsura, Mariko Ikeda, Motoaki Wakiyama, Mutsuko Kukimoto-Niino, Shigeyuki Yokoyama, Yoshikazu Kurosawa, Mikako Shirouzu
Publikováno v:
PLoS ONE, Vol 13, Iss 2, p e0193158 (2018)
Growing numbers of therapeutic antibodies offer excellent treatment strategies for many diseases. Elucidation of the interaction between a potential therapeutic antibody and its target protein by structural analysis reveals the mechanism of action an
Externí odkaz:
https://doaj.org/article/44d4887a9582438299b589d66db0bee7
Autor:
Shisako Shoji, Yutaka Muto, Mariko Ikeda, Fahu He, Kengo Tsuda, Noboru Ohsawa, Ryogo Akasaka, Takaho Terada, Motoaki Wakiyama, Mikako Shirouzu, Shigeyuki Yokoyama
Publikováno v:
FEBS Open Bio, Vol 4, Iss C, Pp 689-703 (2014)
Anaphase-promoting complex or cyclosome (APC/C) is a multisubunit ubiquitin ligase E3 that targets cell-cycle regulators. Cdc20 is required for full activation of APC/C in M phase, and mediates substrate recognition. In vertebrates, Emi2/Erp1/FBXO43
Externí odkaz:
https://doaj.org/article/eebb99b88a1e49e6b0d411a7e38da7e5
Autor:
Motoaki Wakiyama, Koji Takimoto
Publikováno v:
Genes to Cells. 27:579-585
GW182 family proteins are a key component of microRNA-protein complex eliciting translational repression and/or degradation of microRNA-targets. The microRNAs in complex with Argonaute proteins bind to target mRNAs, and GW182 proteins are recruited b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fe77ed5d880bb02c337720aae65c50b
https://doi.org/10.1111/gtc.12974
https://doi.org/10.1111/gtc.12974
Autor:
Kazushige Katsura, Takuhiro Ito, Motoaki Wakiyama, Mutsuko Kukimoto-Niino, Yoshikazu Kurosawa, Chie Takemoto, Takayoshi Matsuda, Mikako Shirouzu, Mariko Ikeda, Shigeyuki Yokoyama
Publikováno v:
PLoS ONE, Vol 13, Iss 2, p e0193158 (2018)
PLoS ONE
PLoS ONE
Growing numbers of therapeutic antibodies offer excellent treatment strategies for many diseases. Elucidation of the interaction between a potential therapeutic antibody and its target protein by structural analysis reveals the mechanism of action an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::795c95062de29bcfb3cbe21115722d47
http://europepmc.org/articles/PMC5819829?pdf=render
http://europepmc.org/articles/PMC5819829?pdf=render
Publikováno v:
Genes to cells : devoted to molecularcellular mechanisms. 23(5)
MicroRNAs are small noncoding RNAs that regulate translation and mRNA stability by binding target mRNAs in complex with Argonaute (AGO) proteins. AGO interacts with a member of the TNRC6 family proteins to form a microRNP complex, which recruits the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6716b51844edbdf70e8df70b061e4aa1
https://pubmed.ncbi.nlm.nih.gov/29626383
https://pubmed.ncbi.nlm.nih.gov/29626383
Inducible protein expression in Drosophila Schneider 2 cells using the lac operator–repressor system
Publikováno v:
Biotechnology Letters. 33:2361-2366
Schneider line 2 cells, derived from Drosophila melanogaster, can be used as a highly versatile gene expression system. Two powerful promoters derived from the actin5C (Ac5) and metallothionein (Mtn) genes are available. The Mtn promoter can be used
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3fcf0ab731a93bcc39771a3de31b5c9
https://doi.org/10.1007/s10529-011-0721-6
https://doi.org/10.1007/s10529-011-0721-6
Autor:
Seisuke Yamashita, Takaho Terada, Peter Güntert, Takanori Kigawa, Takashi Nagata, Shigeyuki Yokoyama, Masahito Kawazoe, Yutaka Muto, Naohiro Kobayashi, Motoaki Wakiyama, Chie Takemoto, Mikako Shirouzu
Publikováno v:
Protein Science. 20:118-130
The TAR RNA-binding Protein (TRBP) is a double-stranded RNA (dsRNA)-binding protein, which binds to Dicer and is required for the RNA interference pathway. TRBP consists of three dsRNA-binding domains (dsRBDs). The first and second dsRBDs (dsRBD1 and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::80601491db20c8759a0c00762acd5101
https://doi.org/10.1002/pro.543
https://doi.org/10.1002/pro.543
Publikováno v:
Protein Science. 19:440-448
Insect cells are useful for the high-yield production of recombinant proteins including chemokines and membrane proteins. In this study, we developed an insect cell-based system for incorporating non-natural amino acids into proteins at specific site
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1f2ab959e71f2612c1d75788ab7de533
https://doi.org/10.1002/pro.322
https://doi.org/10.1002/pro.322
Publikováno v:
Biochemical and Biophysical Research Communications. 343:1067-1071
RNA interference (RNAi) is a fundamental mechanism of gene regulation in a variety of organisms. In Drosophila cells, long double-stranded RNAs (dsRNAs) are processed into 21- to 23-nucleotide double-stranded fragments, termed short interfering RNAs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fda0e75a0878bde5715597cbcc1020d2
https://doi.org/10.1016/j.bbrc.2006.03.076
https://doi.org/10.1016/j.bbrc.2006.03.076
Publikováno v:
Analytical Biochemistry. 427:130-132
To study the functions of RNA-binding proteins independent of their RNA-binding activity, tethering methods have been developed, based on the use of the RNA-binding domain of a well-characterized RNA-binding protein and its target RNA. Two bacterioph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::548c72db1cb5ad1b481bd9cf60994256
https://doi.org/10.1016/j.ab.2012.05.007
https://doi.org/10.1016/j.ab.2012.05.007